Pseudomonas Aeruginosa Mediates Host Necroptosis Through Rhl-Pqs Quorum Sensing Interaction

Overview

Journal Immunohorizons

Specialty Allergy & Immunology

Date 2024 Sep 23

PMID 39312394

Authors

Zihao Liu

Lu Sun

Lupeng Li

Edward A Miao

Amal O Amer

Daniel J Wozniak

Haitao Wen

Affiliations

Soon will be listed here.

Abstract

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen that can cause serious infections in immunocompromised patients. Quorum sensing (QS), a communication system evolved by P. aeruginosa to survey its density, is well acknowledged to be involved in various activities during bacterial infection. Recent studies have revealed the link between P. aeruginosa QS and host innate immune response. Previous evidence suggests that programmed cell death exists in response to P. aeruginosa infection. However, it remains unclear whether QS plays a role in the host programmed cell death process during the infection. In this study, we found that the deficiency of one of QS subsystems, rhl, markedly increased mouse bone marrow macrophage cell death induced by P. aeruginosa, which was accompanied by elevated phosphorylation of RIPK3 and MLKL. This highly increased necroptosis activation was caused by the upregulation of another QS subsystem, pqs, because the deletion of pqs in rhl-deficient P. aeruginosa abolished macrophage necroptosis in vitro and in vivo. In sum, our data highlight the cross-talk between P. aeruginosa QS and host necroptosis, which is executed through the rhl-pqs axis.

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