Ameliorating Effect of Chotosan and Its Active Component, Hook, on Lipopolysaccharide-induced Anxiety-like Behavior in Mice

Overview

Journal Front Pharmacol

Date 2024 Sep 19

PMID 39295939

Authors

Yasumasa Okawa

Soichiro Ushio

Yasuhisa Izushi

Yoshihisa Kitamura

Yoshito Zamami

Toshiaki Sendo

Affiliations

Soon will be listed here.

Abstract

Introduction: In this study, we aimed to examine the effects of chotosan, a traditional Japanese botanical drug, and its active component, hook, on anxiety-like behaviors induced by systemic inflammation in mice.

Methods: To induce systemic inflammation, the mice were treated with lipopolysaccharide (LPS), a bacterial endotoxin. Prior to LPS treatment, the mice were administered chotosan or hook orally each day for 14 days. Anxiety-like behavior of the mice was evaluated using the light-dark test 24 h after LPS treatment.

Results: Repeated administration of chotosan prevented anxiety-like behavior in both normal and LPS-treated mice. Similarly, administration of hook suppressed LPS-induced anxiety-like behavior in mice. Furthermore, treatment with tandospirone, a 5-HT receptor agonist, alleviated anxiety-like behavior in mice, whereas treatment with DOI, a 5-HT receptor agonist, enhanced anxiety-like behavior in mice. LPS treatment significantly increased serotonin (5-HT) receptor mRNA expression in the frontal cortex, whereas 5-HT receptor mRNA expression remained unchanged in the hippocampus. Notably, chotosan significantly suppressed the mRNA expression of 5-HT receptor.

Discussion: These findings indicate that chotosan exerts anxiolytic-like effects in the context of inflammation-induced anxiety, potentially mediated by the inhibition of 5-HT receptor hyperfunction in LPS-treated mice. Consequently, we postulate that chotosan may be effective in managing inflammation-induced anxiety-like behaviors.

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