Comparison of Human Breast Milk Vs Commercial Formula-induced Early Trophic Enteral Nutrition During Postoperative Prolonged Starvation in an Animal Model

Overview

Journal Sci Rep

Specialty Science

Date 2024 Sep 18

PMID 39294167

Authors

Ayca Dogan

Feride Severcan

Ayse Tuzlaci

B Haluk Guvenc

Affiliations

Soon will be listed here.

Abstract

The present study aimed to characterize the changes in macromolecular composition and structure in ileal tissue induced by postoperative prolonged starvation (PS), human breast milk feeding (HM) and commercial formula feeding (CF) for 48 and 72 h (h). Forty-two Wistar albino rats underwent an ileal transection and primary anastomosis and were then divided into six subgroups. Two groups of seven rats were food-deprived for 48 and 72 h with free access to water only in metabolic cages (48 h PS, 72 h PS). Then, two groups of seven rats received early enteral trophic nutrition (EEN) either using HM, and CF at 48 h post-operation (48 h HM, 48 h CF). The other two groups of seven rats received the same trophic enteral nutrition at 72 h post-operation (72 h HM, 72 h CF). An additional seven rats were fed normal rat chow (control), after which the ileal tissues were harvested and freeze-dried overnight. Then sample spectra were recorded by Fourier transform infrared (FTIR) spectroscopy. PS at 48 and 72 h resulted in an increase in the concentration of lipids and a decrease in the concentration of proteins. CF and HM trophic feeding induced a decrease in membrane fluidity and an increase in lipid order. Ileal tissues showed similar compositional and structural changes in lipids and proteins in the PS and CF groups after 48 and 72 h. A marked decrease in nucleic acid concentration was seen in CF at 48 h compared to HM. The human milk feeding groups did not induce any significant alterations and showed compositional and structural data similar to the controls. In conclusion, EEN application seems to be safer when introduced at 48 h rather than 72 h and time of this nutrition is crucial to maintain ileum structure and therefore immunity and well-being. HM-induced trophic nutrition is seen to protect the ileal tissue from significant alterations within lipid and protein compositions, whereas CF caused notable changes. HM is absolutely the best nutritional source for gut health in this animal model.

References

De Luca M, Ioele G, Spatari C, Caruso L, Galasso M, Ragno G . Evaluation of human breastmilk adulteration by combining Fourier transform infrared spectroscopy and partial least square modeling. Food Sci Nutr. 2019; 7(6):2194-2201. PMC: 6593478. DOI: 10.1002/fsn3.1067. View

Wei Y, Pattingre S, Sinha S, Bassik M, Levine B . JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy. Mol Cell. 2008; 30(6):678-88. PMC: 2478643. DOI: 10.1016/j.molcel.2008.06.001. View

Genton L, Cani P, Schrenzel J . Alterations of gut barrier and gut microbiota in food restriction, food deprivation and protein-energy wasting. Clin Nutr. 2014; 34(3):341-9. DOI: 10.1016/j.clnu.2014.10.003. View

Shu X, Kang K, Gu L, Zhang Y . Effect of early enteral nutrition on patients with digestive tract surgery: A meta-analysis of randomized controlled trials. Exp Ther Med. 2016; 12(4):2136-2144. PMC: 5038219. DOI: 10.3892/etm.2016.3559. View

Severcan F, Kazanci N, Zorlu F . Tamoxifen increases membrane fluidity at high concentrations. Biosci Rep. 2000; 20(3):177-84. DOI: 10.1023/a:1005515502954. View

Szalontai B, Nishiyama Y, Gombos Z, Murata N . Membrane dynamics as seen by fourier transform infrared spectroscopy in a cyanobacterium, Synechocystis PCC 6803. The effects of lipid unsaturation and the protein-to-lipid ratio. Biochim Biophys Acta. 2000; 1509(1-2):409-19. DOI: 10.1016/s0005-2736(00)00323-0. View

Yu X, Peng Q, Luo X, An T, Guan J, Wang Z . Effects of Starvation on Lipid Metabolism and Gluconeogenesis in Yak. Asian-Australas J Anim Sci. 2016; 29(11):1593-1600. PMC: 5088379. DOI: 10.5713/ajas.15.0868. View

Abela G . The potential benefits and harms of early feeding post-surgery: a literature review. Int Wound J. 2017; 14(5):870-873. PMC: 7949951. DOI: 10.1111/iwj.12750. View

Garwolinska D, Hewelt-Belka W, Kot-Wasik A, Sundekilde U . Nuclear Magnetic Resonance Metabolomics Reveals Qualitative and Quantitative Differences in the Composition of Human Breast Milk and Milk Formulas. Nutrients. 2020; 12(4). PMC: 7230615. DOI: 10.3390/nu12040921. View

10.

Chappell V, Thompson M, Jeschke M, Chung D, Thompson J, Wolf S . Effects of incremental starvation on gut mucosa. Dig Dis Sci. 2003; 48(4):765-9. DOI: 10.1023/a:1022849112100. View

11.

Mozaheb N, Van Der Smissen P, Opsomer T, Mignolet E, Terrasi R, Paquot A . Contribution of Membrane Vesicle to Reprogramming of Bacterial Membrane Fluidity in Pseudomonas aeruginosa. mSphere. 2022; 7(3):e0018722. PMC: 9241526. DOI: 10.1128/msphere.00187-22. View

12.

BROWN H, LEVINE M, Lipkin M . Inhibition of intestinal epithelial cell renewal and migration induced by starvation. Am J Physiol. 1963; 205(5):868-72. DOI: 10.1152/ajplegacy.1963.205.5.868. View

13.

Robinson D, Caplan M . Linking fat intake, the intestinal microbiome, and necrotizing enterocolitis in premature infants. Pediatr Res. 2014; 77(1-2):121-6. DOI: 10.1038/pr.2014.155. View

14.

Janmey P, Kinnunen P . Biophysical properties of lipids and dynamic membranes. Trends Cell Biol. 2006; 16(10):538-46. DOI: 10.1016/j.tcb.2006.08.009. View

15.

. Breastfeeding and the use of human milk. Pediatrics. 2012; 129(3):e827-41. DOI: 10.1542/peds.2011-3552. View

16.

Krafft C, Steiner G, Beleites C, Salzer R . Disease recognition by infrared and Raman spectroscopy. J Biophotonics. 2009; 2(1-2):13-28. DOI: 10.1002/jbio.200810024. View

17.

Gok S, Kuzmenko O, Babinskyi A, Severcan F . Vitamin E Derivative with Modified Side Chain Induced Apoptosis by Modulating the Cellular Lipids and Membrane Dynamics in MCF7 Cells. Cell Biochem Biophys. 2021; 79(2):271-287. DOI: 10.1007/s12013-020-00961-y. View

18.

Ekingen G, Ceran C, Guvenc B, Tuzlaci A, Kahraman H . Early enteral feeding in newborn surgical patients. Nutrition. 2005; 21(2):142-6. DOI: 10.1016/j.nut.2004.10.003. View

19.

Karabiyik C, Vicinanza M, Son S, Rubinsztein D . Glucose starvation induces autophagy via ULK1-mediated activation of PIKfyve in an AMPK-dependent manner. Dev Cell. 2021; 56(13):1961-1975.e5. DOI: 10.1016/j.devcel.2021.05.010. View

20.

Ergun S, Demir P, Uzbay T, Severcan F . Agomelatine strongly interacts with zwitterionic DPPC and charged DPPG membranes. Biochim Biophys Acta. 2014; 1838(11):2798-806. DOI: 10.1016/j.bbamem.2014.07.025. View