Genetic Dissection of Drosophila Adenylate Cyclase

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Sep 1

PMID 3929247

Citations 19

Authors

M S Livingstone

Affiliations

Soon will be listed here.

Abstract

The Drosophila learning mutant rutabaga is missing calcium/calmodulin activation of adenylate cyclase (EC4.6.1.1). The mutation was mapped at a finer resolution to X chromosome bands 12F5-7. By comparing wild-type and mutant cyclase activities, the relative responsiveness of the calcium-sensitive and calcium-insensitive components to different ligands could be determined; the calcium-sensitive fraction of the total cyclase activity was significantly less responsive to guanyl nucleotides and monoamines. The results suggest that the component of cyclase activity that is stimulated by calcium/calmodulin, possibly a genetically distinct catalytic subunit, is not coupled to the G subunit or the G subunit/monoamine receptor complex.

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