Identification of High-performing Antibodies for SPARC-related Modular Calcium-binding Protein 1 (SMOC-1) for Use in Western Blot and Immunoprecipitation

Overview

Journal F1000Res

Specialties Biomedical Engineering
Science

Date 2024 Sep 18

PMID 39291144

Authors

Riham Ayoubi

Sara Gonzalez Bolivar

Michael Nicouleau

Kathleen Southern

Carl Laflamme

Affiliations

Soon will be listed here.

Abstract

SPARC-related modular calcium-binding protein 1, otherwise known as SMOC-1, is a secreted glycoprotein involved in various cell biological processes including cell-matrix interactions, osteoblast differentiation, embryonic development, and homeostasis. SMOC-1 was found to be elevated in asymptomatic Alzheimer's disease (AD) patient cortex as well as being enriched in amyloid plaques and in AD patientcerebrospinal fluid, arguing for SMOC-1 as a promising biomarker for AD. Having access to high-quality SMOC-1 antibodies is crucial for the scientific community. It can ensure the consistency and reliability of SMOC-1 research, and further the exploration of its potential as both a therapeutic target or diagnostic marker.. In this study, we characterized seven SMOC-1 commercial antibodies for Western blot and immunoprecipitation, using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified successful antibodies in the tested applications and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.

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