Genome‑wide Analysis of Cotton SCAMP Genes and Functional Characterization of GhSCAMP2 and GhSCAMP4 in Salt Tolerance

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2024 Sep 17

PMID 39289615

Authors

Zhaojie He

XiaoHu Ma

Qian-Hao Zhu

Shuaishuai Cheng

Feng Liu

Tao Zhang

Caixia Zhang

Jianbin Li

Xianpeng Xiong

Jie Sun

Affiliations

Soon will be listed here.

Abstract

Background: Secretory carrier membrane proteins (SCAMPs) form a family of integral membrane proteins and play a crucial role in mediating exocytosis in both animals and plants. While SCAMP genes have been studied in several plant species, their functions in cotton, particularly in response to abiotic stress, have not yet been reported.

Results: In this study, a total of 53 SCAMP genes were identified in G. arboreum, G. raimondii, G. hirsutum, and G. barbadense. These genes were classified into five groups based on a phylogenetic analysis with SCAMPs from Arabidopsis thaliana. The main factor driving the expansion of the SCAMP gene family in G. hirsutum is tandem and segmental duplication events. Using MEME, in addition to the conserved SCAMP domain, we identified 3-13 other domains in each GhSCAMP. The cis-element analysis suggested that GhSCAMPs were widely involved in cotton growth and development, and responses to abiotic stresses. RNA sequencing (RNA-Seq) and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) results showed that most GhSCAMPs were expressed highly in many tissues and had differential expression responses to drought, cold, and heat stresses. Knock-down of GhSCAMP2 and GhSCAMP4 by virus-induced gene silencing (VIGS) lead to a salt-sensitive phenotype and had a lower content of CAT, POD, and SOD.

Conclusions: This study identified SCAMP genes in four cotton species, enhancing our understanding of the potential biological functions of SCAMPs. Additionally, we demonstrated that GhSCAMP2 and GhSCAMP4 positively regulate cotton tolerance to salt stress.

Citing Articles

Correction: Genome-wide analysis of cotton SCAMP genes and functional characterization of GhSCAMP2 and GhSCAMP4 in salt tolerance.

He Z, Ma X, Zhu Q, Cheng S, Liu F, Zhang T BMC Plant Biol. 2024; 24(1):932.

PMID: 39375587 PMC: 11459828. DOI: 10.1186/s12870-024-05655-8.

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