Cellular Location of Origin and Terminus of Replication in Bacillus Subtilis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1985 Sep 1

PMID 3928600

Citations 2

Authors

E M Sonnenfeld

A L Koch

R J Doyle

Affiliations

Soon will be listed here.

Abstract

The origin of replication of Bacillus subtilis 168 trp thy dna-1 (temperature-sensitive initiation mutant) was labeled with [3H]thymidine. Analysis of labeled cells by autoradiography revealed that most of the radioactivity was associated with cell pole areas. To label the terminus, cells that had initiated were treated with chloramphenicol to inhibit cell growth and division but to allow continued DNA synthesis. These cells were then labeled with [3H]thymidine at a time when chromosome replication was nearly complete. The distribution of radioactivity was similar to that observed in origin-labeled cells. In contrast, exponentially growing cells that were labeled for a brief time at the permissive temperature showed a random distribution of radioactivity. These data indicate that the origin and terminus of replication are located at cell poles.

Citing Articles

Chromosome and cell wall segregation in Streptococcus faecium ATCC 9790.

Higgins M, Glaser D, Dicker D, Zito E J Bacteriol. 1989; 171(1):349-52.

PMID: 2914850 PMC: 209594. DOI: 10.1128/jb.171.1.349-352.1989.

To shape a cell: an inquiry into the causes of morphogenesis of microorganisms.

Harold F Microbiol Rev. 1990; 54(4):381-431.

PMID: 2128368 PMC: 372787. DOI: 10.1128/mr.54.4.381-431.1990.

References

Chai N, Lark K . Segregation of deoxyribonucleic acid in bacteria: association of the segregating unit with the cell envelope. J Bacteriol. 1967; 94(2):415-21. PMC: 315056. DOI: 10.1128/jb.94.2.415-421.1967. View

Mendelson N . A model of bacterial DNA segregation based upon helical geometry. J Theor Biol. 1985; 112(1):25-39. DOI: 10.1016/s0022-5193(85)80115-6. View

Fan D, Pelvit M, Cunningham W . Structural difference between walls from ends and sides of the rod-shaped bacterium Bacillus subtilis. J Bacteriol. 1972; 109(3):1266-72. PMC: 247351. DOI: 10.1128/jb.109.3.1266-1272.1972. View

White K, Sueoka N . Temperature-sensitive DNA synthesis mutants of Bacillus subtilis--appendix: theory of density transfer for symmetric chromosome replication. Genetics. 1973; 73(2):185-214. PMC: 1212886. DOI: 10.1093/genetics/73.2.185. View

Fan D, Beckman B . Structural difference between walls from hemispherical caps and partial septa of Bacillus subtilis. J Bacteriol. 1973; 114(2):790-7. PMC: 251840. DOI: 10.1128/jb.114.2.790-797.1973. View

Sueoka N, Hammers J . Isolation of DNA-membrane complex in Bacillus subtilis. Proc Natl Acad Sci U S A. 1974; 71(12):4787-91. PMC: 433982. DOI: 10.1073/pnas.71.12.4787. View

Sargent M . Control of cell length in Bacillus subtilis. J Bacteriol. 1975; 123(1):7-19. PMC: 235685. DOI: 10.1128/jb.123.1.7-19.1975. View

Archibald A, Coapes H . Bacteriophage SP50 as a marker for cell wall growth in Bacillus subtilis. J Bacteriol. 1976; 125(3):1195-206. PMC: 236200. DOI: 10.1128/jb.125.3.1195-1206.1976. View

Neely J, COMBS J . Variation in the autoradiographic technique: I. Emulsion-developer combinations assessed by photometric measurement of single silver grains. J Histochem Cytochem. 1976; 24(10):1057-64. DOI: 10.1177/24.10.977937. View

10.

Sargent M . Surface extension and the cell cycle in prokaryotes. Adv Microb Physiol. 1978; 18:105-76. DOI: 10.1016/s0065-2911(08)60416-6. View

11.

Beeson J, Sueoka N . Membrane enrichment of genetic markers close to the origin and terminus during the deoxyribonucleic acid replication cycle in Bacillus subtilis. J Bacteriol. 1979; 139(3):911-6. PMC: 218038. DOI: 10.1128/jb.139.3.911-916.1979. View

12.

Meacock P, Cohen S . Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance. Cell. 1980; 20(2):529-42. DOI: 10.1016/0092-8674(80)90639-x. View

13.

Winston S, Sueoka N . DNA-membrane association is necessary for initiation of chromosomal and plasmid replication in Bacillus subtilis. Proc Natl Acad Sci U S A. 1980; 77(5):2834-8. PMC: 349499. DOI: 10.1073/pnas.77.5.2834. View

14.

Doyle R, Streips U, Imada S, Fan V, Brown W . Genetic transformation with cell wall-associated deoxyribonucleic acid in Bacillus subtilis. J Bacteriol. 1980; 144(3):957-66. PMC: 294758. DOI: 10.1128/jb.144.3.957-966.1980. View

15.

Sandler N, Keynan A . Cell wall synthesis and initiation of deoxyribonucleic acid replication in Bacillus subtilis. J Bacteriol. 1981; 148(2):443-9. PMC: 216225. DOI: 10.1128/jb.148.2.443-449.1981. View

16.

Hendrickson W, Kusano T, Yamaki H, Balakrishnan R, King M, Murchie J . Binding of the origin of replication of Escherichia coli to the outer membrane. Cell. 1982; 30(3):915-23. DOI: 10.1016/0092-8674(82)90296-3. View

17.

Schlaeppi J, KARAMATA D . Cosegregation of cell wall and DNA in Bacillus subtilis. J Bacteriol. 1982; 152(3):1231-40. PMC: 221630. DOI: 10.1128/jb.152.3.1231-1240.1982. View

18.

Ogura T, Hiraga S . Partition mechanism of F plasmid: two plasmid gene-encoded products and a cis-acting region are involved in partition. Cell. 1983; 32(2):351-60. DOI: 10.1016/0092-8674(83)90454-3. View

19.

Doyle R, Koch A, Carstens P . Cell wall-DNA association in Bacillus subtilis. J Bacteriol. 1983; 153(3):1521-7. PMC: 221804. DOI: 10.1128/jb.153.3.1521-1527.1983. View

20.

Austin S, Abeles A . Partition of unit-copy miniplasmids to daughter cells. I. P1 and F miniplasmids contain discrete, interchangeable sequences sufficient to promote equipartition. J Mol Biol. 1983; 169(2):353-72. DOI: 10.1016/s0022-2836(83)80055-2. View