Evaluation of Magnetic Hyperthermia, Drug Delivery and Biocompatibility (bone Cell Adhesion and Zebrafish Assays) of Trace Element Co-doped Hydroxyapatite Combined with Mn-Zn Ferrite for Bone Tissue Applications

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2024 Sep 17

PMID 39285891

Authors

Tanatsaparn Tithito

Siwapech Sillapaprayoon

Varissara Chantho

Wittaya Pimtong

Jirawan Thongbunchoo

Narattaphol Charoenphandhu

Nateetip Krishnamra

Nararat Yong

Aurachat Lert-Itthiporn

Weerakanya Maneeprakorn

Weeraphat Pon-On

Affiliations

Soon will be listed here.

Abstract

The treatment and regeneration of bone defects, especially tumor-induced defects, is an issue in clinical practice and remains a major challenge for bone substitute material invention. In this research, a composite material of biomimetic bone-like apatite based on trace element co-doped apatite (Ca M (PO)(CO)(OH); M = trace elements of Mg, Fe, Zn, Mn, Cu, Ni, Mo, Sr and BO ; THA)-integrated-biocompatible magnetic Mn-Zn ferrite ((Mn, Zn)FeO nanoparticles, BioMags) called THAiBioMags was prepared a co-precipitation method. Its characteristics, , physical properties, hyperthermia performance, ion/drug delivery, were investigated using osteoblasts (bone-forming cells) and using zebrafish. The synthesized THAiBioMags particles revealed superparamagnetic behaviour at room temperature. Under the influence of an alternating magnetic field, THAiBioMags particles demonstrated a change in temperature, indicating their potential for magnetic hyperthermia, in which THAiBioMags further exhibited a specific absorption rate (SAR) value of 9.44 W g ( = 44 A, = 6.03 kA m and = 130 kHz). In addition, the as-prepared particles demonstrated sustained ion/drug (doxorubicin (DOX)) release under physiological pH conditions. Biological assay analysis revealed that THAiBioMags exhibited no toxicity towards osteoblast cells and demonstrated excellent cell adhesion properties. studies employing an embryonic zebrafish model showed the non-toxic nature of the synthesized THAiBioMags particles, as revealed by evaluation of the survivability, hatching rate, and embryonic morphology. These results could potentially lead to the design and fabrication of magnetic scaffolds to be used in therapeutic treatment and bone regeneration.

Citing Articles

Potential Molecular Interactions and In Vitro Hyperthermia, Thermal, and Magnetic Studies of Bioactive Nickel-Doped Hydroxyapatite Thin Films.

Asghar M, Ghazanfar U, Rizwan M, Manan M, Baig A, Qaiser M Int J Mol Sci. 2025; 26(3).

PMID: 39940863 PMC: 11817106. DOI: 10.3390/ijms26031095.

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