Synergistic Potential of Stem Cells and Microfluidics in Regenerative Medicine

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2024 Sep 16

PMID 39285093

Authors

Resmi Rajalekshmi

Devendra K Agrawal

Affiliations

Soon will be listed here.

Abstract

Regenerative medicine has immense potential to revolutionize healthcare by using regenerative capabilities of stem cells. Microfluidics, a cutting-edge technology, offers precise control over cellular microenvironments. The integration of these two fields provides a deep understanding of stem cell behavior and enables the development of advanced therapeutic strategies. This critical review explores the use of microfluidic systems to culture and differentiate stem cells with precision. We examined the use of microfluidic platforms for controlled nutrient supply, mechanical stimuli, and real-time monitoring, providing an unprecedented level of detail in studying cellular responses. The convergence of stem cells and microfluidics holds immense promise for tissue repair, regeneration, and personalized medicine. It offers a unique opportunity to revolutionize the approach to regenerative medicine, facilitating the development of advanced therapeutic strategies and enhancing healthcare outcomes.

References

Hanna H, Mir L, Andre F . In vitro osteoblastic differentiation of mesenchymal stem cells generates cell layers with distinct properties. Stem Cell Res Ther. 2018; 9(1):203. PMC: 6063016. DOI: 10.1186/s13287-018-0942-x. View

Akther F, Little P, Li Z, Nguyen N, Ta H . Hydrogels as artificial matrices for cell seeding in microfluidic devices. RSC Adv. 2022; 10(71):43682-43703. PMC: 9058401. DOI: 10.1039/d0ra08566a. View

Mackay-Sim A, Silburn P . Stem cells and genetic disease. Cell Prolif. 2008; 41 Suppl 1:85-93. PMC: 6496804. DOI: 10.1111/j.1365-2184.2008.00487.x. View

Gao Q, Wang L, Wang S, Huang B, Jing Y, Su J . Bone Marrow Mesenchymal Stromal Cells: Identification, Classification, and Differentiation. Front Cell Dev Biol. 2022; 9:787118. PMC: 8762234. DOI: 10.3389/fcell.2021.787118. View

Cohen P, Luquet E, Pletenka J, Leonard A, Warter E, Gurchenkov B . Engineering 3D micro-compartments for highly efficient and scale-independent expansion of human pluripotent stem cells in bioreactors. Biomaterials. 2023; 295:122033. DOI: 10.1016/j.biomaterials.2023.122033. View

Song H, Rosano J, Wang Y, Garson C, Prabhakarpandian B, Pant K . Continuous-flow sorting of stem cells and differentiation products based on dielectrophoresis. Lab Chip. 2015; 15(5):1320-8. PMC: 8385543. DOI: 10.1039/c4lc01253d. View

Kwon S, Kwon Y, Lee T, Park G, Kim J . Recent advances in stem cell therapeutics and tissue engineering strategies. Biomater Res. 2019; 22:36. PMC: 6299977. DOI: 10.1186/s40824-018-0148-4. View

Pattanayak P, Singh S, Gulati M, Vishwas S, Kapoor B, Chellappan D . Microfluidic chips: recent advances, critical strategies in design, applications and future perspectives. Microfluid Nanofluidics. 2021; 25(12):99. PMC: 8547131. DOI: 10.1007/s10404-021-02502-2. View

Volarevic V, Markovic B, Gazdic M, Volarevic A, Jovicic N, Arsenijevic N . Ethical and Safety Issues of Stem Cell-Based Therapy. Int J Med Sci. 2018; 15(1):36-45. PMC: 5765738. DOI: 10.7150/ijms.21666. View

10.

Knoepfler P . Deconstructing stem cell tumorigenicity: a roadmap to safe regenerative medicine. Stem Cells. 2009; 27(5):1050-6. PMC: 2733374. DOI: 10.1002/stem.37. View

11.

Kerk Y, Jameel A, Xing X, Zhang C . Recent advances of integrated microfluidic suspension cell culture system. Eng Biol. 2023; 5(4):103-119. PMC: 9996741. DOI: 10.1049/enb2.12015. View

12.

Rackus D, Riedel-Kruse I, Pamme N . "Learning on a chip:" Microfluidics for formal and informal science education. Biomicrofluidics. 2019; 13(4):041501. PMC: 6697029. DOI: 10.1063/1.5096030. View

13.

Ashton R, Keung A, Peltier J, Schaffer D . Progress and prospects for stem cell engineering. Annu Rev Chem Biomol Eng. 2012; 2:479-502. PMC: 5991629. DOI: 10.1146/annurev-chembioeng-061010-114105. View

14.

Liu G, Wu Y, Kong F, Ma S, Fu L, Geng J . BMSCs differentiated into neurons, astrocytes and oligodendrocytes alleviated the inflammation and demyelination of EAE mice models. PLoS One. 2021; 16(5):e0243014. PMC: 8118321. DOI: 10.1371/journal.pone.0243014. View

15.

Daniszewski M, Crombie D, Henderson R, Liang H, Wong R, Hewitt A . Automated Cell Culture Systems and Their Applications to Human Pluripotent Stem Cell Studies. SLAS Technol. 2017; 23(4):315-325. DOI: 10.1177/2472630317712220. View

16.

Kato S, Carlson D, Shen A, Guo Y . Twisted fiber microfluidics: a cutting-edge approach to 3D spiral devices. Microsyst Nanoeng. 2024; 10:14. PMC: 10800335. DOI: 10.1038/s41378-023-00642-9. View

17.

Jammes F, Maerkl S . How single-cell immunology is benefiting from microfluidic technologies. Microsyst Nanoeng. 2021; 6:45. PMC: 8433390. DOI: 10.1038/s41378-020-0140-8. View

18.

Pillai S, Kwan J, Yaziji F, Yu H, Tran S . Mapping the Potential of Microfluidics in Early Diagnosis and Personalized Treatment of Head and Neck Cancers. Cancers (Basel). 2023; 15(15). PMC: 10417175. DOI: 10.3390/cancers15153894. View

19.

Wei L, Li W, Entcheva E, Li Z . Microfluidics-enabled 96-well perfusion system for high-throughput tissue engineering and long-term all-optical electrophysiology. Lab Chip. 2020; 20(21):4031-4042. PMC: 7680692. DOI: 10.1039/d0lc00615g. View

20.

Liu M, Liu N, Zang R, Li Y, Yang S . Engineering stem cell niches in bioreactors. World J Stem Cells. 2013; 5(4):124-35. PMC: 3812517. DOI: 10.4252/wjsc.v5.i4.124. View