Mechanism of Sensory Perception Unveiled by Simultaneous Measurement of Membrane Voltage and Intracellular Calcium

Overview

Journal Commun Biol

Specialty Biology

Date 2024 Sep 16

PMID 39284959

Authors

Terumasa Tokunaga

Noriko Sato

Mary Arai

Takumi Nakamura

Takeshi Ishihara

Affiliations

Soon will be listed here.

Abstract

Measuring neuronal activity is important for understanding neuronal function. Ca imaging by genetically encoded calcium indicators (GECIs) is a powerful way to measure neuronal activity. Although it revealed important aspects of neuronal function, measuring the neuronal membrane voltage is important to understand neuronal function as it triggers neuronal activation. Recent progress of genetically encoded voltage indicators (GEVIs) enabled us fast and precise measurements of neuronal membrane voltage. To clarify the relation of the membrane voltage and intracellular Ca, we analyzed neuronal activities of olfactory neuron AWA in Caenorhabditis elegans by GCaMP6f (GECI) and paQuasAr3 (GEVI) responding to odorants. We found that the membrane voltage encodes the stimuli change by the timing and the duration by the weak semi-stable depolarization. However, the change of the intracellular Ca encodes the strength of the stimuli. Furthermore, ODR-3, a G-protein alpha subunit, was shown to be important for stabilizing the membrane voltage. These results suggest that the combination of calcium and voltage imaging provides a deeper understanding of the information in neural circuits.

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