Untargeted Metabolomics and Gut Microbiota Modulation Study of Fermented Brown Rice for Obesity

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2024 Sep 16

PMID 39281900

Authors

Kaliyan Barathikannan

Ramachandran Chelliah

Selvakumar Vijayalakshmi

Fred Kwame Ofosu

Su-Jung Yeon

Deuk-Sik Lee

Jong-Soon Park

Nam-Hyeon Kim

Deog-Hwan Oh

Affiliations

Soon will be listed here.

Abstract

Obesity or excess adipose tissue mass increases the risk of heart disease, hypertension, and diabetes. Obesity might be prevented by consuming plant-based probiotic fermented foods. This study aimed to determine whether adding MNL5 to fermented brown rice (FBR) enhances its metabolites, lipase activity, and antioxidant efficiency. UHPLC-Q-TOF-MS/MS analysis revealed significant changes in untargeted metabolite profiles, while, compared with those of raw brown rice (RBR), FBR contained more antioxidant and lipase inhibitors. We evaluated the FBR in HFD (high-fat-diet)-induced obese mice by employing biochemical, histological, gut microbiome, and serum metabolomics approaches. FBR MD (250 mg/kg) decreased body weight (BW) and fat content compared with RBR. With subsequent FBR MD, mice fed a HFD may have reduced serum lipid levels. A HFD with a mid-dose FBR improved the gut microbiota diversity, composition, and structure; reduced the abundance of obesity-related genera such as , , and ; and promoted the abundance of beneficial genera such as , , and , which are inversely correlated with BW, total cholesterol, TG, LDL-C, and HDL-C. In addition, FBR MD has been associated with increased levels of palmitic acid, EPA, oleic acid, α-linolenic acid, indole, dodecanoic acid, and amino acids. FBR, in its entirety, has exhibited promise as a functional material for ameliorating obesity.

References

Baruah R, Ray M, Halami P . Preventive and therapeutic aspects of fermented foods. J Appl Microbiol. 2022; 132(5):3476-3489. DOI: 10.1111/jam.15444. View

Tyagi A, Shabbir U, Chelliah R, Daliri E, Chen X, Oh D . Fermented Brown Rice: A Product with Enhanced Bioactive Compounds and Antioxidant Potential. Antioxidants (Basel). 2021; 10(7). PMC: 8301027. DOI: 10.3390/antiox10071077. View

Rodrigues C, Salgueiro W, Bianchini M, Veit J, Puntel R, Emanuelli T . L. (chia) seeds oil extracts reduce lipid accumulation and produce stress resistance in . Nutr Metab (Lond). 2018; 15:83. PMC: 6260566. DOI: 10.1186/s12986-018-0317-4. View

Naowaboot J, Piyabhan P, Munkong N, Parklak W, Pannangpetch P . Ferulic acid improves lipid and glucose homeostasis in high-fat diet-induced obese mice. Clin Exp Pharmacol Physiol. 2015; 43(2):242-50. DOI: 10.1111/1440-1681.12514. View

Ge Y, Shi Y, Wei C, Uthamapriya R, Wu Y, Cao L . The effects of quinoa bran dietary fiber on glucose and lipid metabolism and hepatic transcriptome in obese rats. J Sci Food Agric. 2023; 104(5):2692-2703. DOI: 10.1002/jsfa.13154. View

Tham K, Abdul Ghani R, Cua S, Deerochanawong C, Fojas M, Hocking S . Obesity in South and Southeast Asia-A new consensus on care and management. Obes Rev. 2022; 24(2):e13520. PMC: 10078503. DOI: 10.1111/obr.13520. View

Zhang M, Yang T, Li R, Ren K, Li J, He M . Gut microbiota of Suncus murinus, a naturally obesity-resistant animal, improves the ecological diversity of the gut microbiota in high-fat-diet-induced obese mice. PLoS One. 2023; 18(11):e0293213. PMC: 10664932. DOI: 10.1371/journal.pone.0293213. View

Galimberti A, Bruno A, Agostinetto G, Casiraghi M, Guzzetti L, Labra M . Fermented food products in the era of globalization: tradition meets biotechnology innovations. Curr Opin Biotechnol. 2020; 70:36-41. DOI: 10.1016/j.copbio.2020.10.006. View

Barathikannan K, Chelliah R, Elahi F, Tyagi A, Selvakumar V, Agastian P . Anti-Obesity Efficacy of MNL5 in Gut Model. Int J Mol Sci. 2022; 23(3). PMC: 8835910. DOI: 10.3390/ijms23031276. View

10.

Kang H, Kim J, Kim J, Bang W, Choi H, Kim N . Unlocking the Protective Potential of Upper Respiratory Infection Treatment Histories against Alzheimer's Disease: A Korean Adult Population Study. J Clin Med. 2024; 13(1). PMC: 10780066. DOI: 10.3390/jcm13010260. View

11.

Camargo A, Meneses M, Perez-Martinez P, Delgado-Lista J, Jimenez-Gomez Y, Cruz-Teno C . Dietary fat differentially influences the lipids storage on the adipose tissue in metabolic syndrome patients. Eur J Nutr. 2013; 53(2):617-26. DOI: 10.1007/s00394-013-0570-2. View

12.

Lee M, Yun Y, Choi E, Song J, Kang J, Kim D . Anti-obesity effect of vegetable juice fermented with lactic acid bacteria isolated from kimchi in C57BL/6J mice and human mesenchymal stem cells. Food Funct. 2023; 14(3):1349-1356. DOI: 10.1039/d2fo02998g. View

13.

Tyagi A, Lim M, Kim N, Barathikannan K, Vijayalakshmi S, Elahi F . Quantification of Amino Acids, Phenolic Compounds Profiling from Nine Rice Varieties and Their Antioxidant Potential. Antioxidants (Basel). 2022; 11(5). PMC: 9137474. DOI: 10.3390/antiox11050839. View

14.

Rigaudiere J, Jouve C, Capel F, Patrac V, Miguel B, Tournadre A . An experimental model of western diet in female Wistar rats leads to cardiac hypoxia related to a stimulated contractility. J Physiol Biochem. 2024; 80(2):287-302. DOI: 10.1007/s13105-023-01003-w. View

15.

Wang R, Wang L, Wang S, Wang J, Su C, Zhang L . Phenolics from noni (Morinda citrifolia L.) fruit alleviate obesity in high fat diet-fed mice via modulating the gut microbiota and mitigating intestinal damage. Food Chem. 2022; 402:134232. DOI: 10.1016/j.foodchem.2022.134232. View

16.

Landgraf K, Kloting N, Gericke M, Maixner N, Guiu-Jurado E, Scholz M . The Obesity-Susceptibility Gene TMEM18 Promotes Adipogenesis through Activation of PPARG. Cell Rep. 2020; 33(3):108295. DOI: 10.1016/j.celrep.2020.108295. View

17.

Li M, Kang S, Huang K, Tong T . Dietary Supplementation of Methyl Cedryl Ether Ameliorates Adiposity in High-Fat Diet-Fed Mice. Nutrients. 2023; 15(3). PMC: 9919899. DOI: 10.3390/nu15030788. View

18.

Barathikannan K, Chelliah R, Yeon S, Tyagi A, Elahi F, Vijayalakshmi S . Untargeted metabolomics of fermented onion (Allium cepa L) using UHPLC Q-TOF MS/MS reveals anti-obesity metabolites and in vivo efficacy in Caenorhabditis elegans. Food Chem. 2022; 404(Pt B):134710. DOI: 10.1016/j.foodchem.2022.134710. View

19.

Zhao X, Fu Z, Yao M, Cao Y, Zhu T, Mao R . Mulberry ( L.) leaf polysaccharide ameliorates insulin resistance- and adipose deposition-associated gut microbiota and lipid metabolites in high-fat diet-induced obese mice. Food Sci Nutr. 2022; 10(2):617-630. PMC: 8825736. DOI: 10.1002/fsn3.2689. View

20.

Jemil I, Mora L, Nasri R, Abdelhedi O, Aristoy M, Hajji M . A peptidomic approach for the identification of antioxidant and ACE-inhibitory peptides in sardinelle protein hydrolysates fermented by Bacillus subtilis A26 and Bacillus amyloliquefaciens An6. Food Res Int. 2017; 89(Pt 1):347-358. DOI: 10.1016/j.foodres.2016.08.020. View