Rapid Assessment of Metabolomic Fingerprinting of Recycled Sunflower By-Products Via DART-HRMS

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2024 Sep 14

PMID 39274940

Authors

Carmela Zacometti

Anna Lante

Miluska Cisneros

Andrea Massaro

Dasha Mihaylova

Vesela Chalova

Albert Krastanov

Hristo Kalaydzhiev

Giorgia Riuzzi

Alessandra Tata

Severino Segato

Affiliations

Soon will be listed here.

Abstract

To comply with a more circular and environmentally friendly European common agricultural policy, while also valorising sunflower by-products, an ultrasound assisted extraction (UAE) was tested to optimise ethanol-wash solutes (EWS). Furthermore, the capabilities of DART-HRMS as a rapid and cost-effective tool for determining the biochemical changes after valorisation of these defatted sunflower EWS were investigated. Three batches of EWS were doubly processed into optimised EWS (OEWS) samples, which were analysed via DART-HRMS. Then, the metabolic profiles were submitted to a univariate analysis followed by a partial least square discriminant analysis (PLS-DA) allowing the identification of the 15 most informative ions. The assessment of the metabolomic fingerprinting characterising EWS and OEWS resulted in an accurate and well-defined spatial clusterization based on the retrieved pool of informative ions. The outcomes highlighted a significantly higher relative abundance of phenolipid hydroxycinnamoyl-glyceric acid and a lower incidence of free fatty acids and diglycerides due to the ultrasound treatment. These resulting biochemical changes might turn OEWS into a natural antioxidant supplement useful for controlling lipid oxidation and to prolong the shelf-life of foods and feeds. A standardised processing leading to a selective concentration of the desirable bioactive compounds is also advisable.

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