Modifying the Ambient Light Spectrum Using LED Lamps Alters the Phenolic Profile of Hydroponically Grown Greenhouse Lettuce Plants Without Affecting Their Agronomic Characteristics

Overview

Journal Plants (Basel)

Date 2024 Sep 14

PMID 39273950

Authors

Cristian Hernandez-Adasme

Herman Silva

Alvaro Pena

Maria Gabriela Vargas-Martinez

Carolina Salazar-Parra

Bo Sun

Victor Escalona Contreras

Affiliations

Soon will be listed here.

Abstract

The growth and development of green lettuce plants can be modulated by the prevailing light conditions around them. The aim of this study was to evaluate the effect of ambient light enrichment with different LED light spectra on agronomic characteristics, polyphenol concentration and relative gene expression of enzymes associated with polyphenol formation in 'Levistro' lettuce grown hydroponically in a Nutrient Film Technique (NFT) system for 28 days in a greenhouse. The spectra (blue:green:red:far-red) and red:blue (R:B) ratios obtained by enriching ambient light with Blue (B), White (W), Blue-Red (BR) and Red (R) LED light were B: 47:22:21:10, 0.5:1; W: 30:38:23:9, 0.8:1; BR: 33:15:44:8, 1.3:1 and R: 16:16:60:8, 3.8:1, respectively, and photosynthetically active radiation (PAR) under the different treatments, measured at midday, ranged from 328 to 336 µmoles m s. The resulting daily light integral (DLI) was between 9.1 and 9.6 mol m day. The photoperiod for all enrichment treatments was 12 h of light. The control was ambient greenhouse light (25:30:30:15; R:B = 1.2:1; PAR = 702 µmoles m s; DLI = 16.9 mol m day; photoperiod = 14.2 h of light). Fresh weight (FW) and dried weight percentage (DWP) were similar among the enrichment treatments and the control. The leaf number increased significantly under BR and R compared to B lights. The relative index of chlorophyll concentration (RIC) increased as plants grew and was similar among the enrichment treatments and the control. On the other hand, the concentration of chlorogenic acid and chicoric acid increased under BR and B lights, which was consistent with the higher relative expression of the enzyme gene. In view of the results, it is inferred that half of the PAR or DLI is sufficient to achieve normal growth and development of 'Levistro' lettuce plants, suggesting a more efficient use of light energy under the light enrichment treatments. On the other hand, the blue and combined blue-red lights promoted the accumulation of phenolic compounds in the leaves of 'Levistro' lettuce plants.

References

Naoya Fukuda M, Yoshida H, Kusano M . Effects of light quality, photoperiod, CO concentration, and air temperature on chlorogenic acid and rutin accumulation in young lettuce plants. Plant Physiol Biochem. 2022; 186:290-298. DOI: 10.1016/j.plaphy.2022.07.010. View

Siwach P, Gill A . Micropropagation of Ficus religiosa L. via leaf explants and comparative evaluation of acetylcholinesterase inhibitory activity in the micropropagated and conventionally grown plants. 3 Biotech. 2017; 4(5):477-491. PMC: 4162900. DOI: 10.1007/s13205-013-0175-8. View

Bian Z, Yang Q, Liu W . Effects of light quality on the accumulation of phytochemicals in vegetables produced in controlled environments: a review. J Sci Food Agric. 2014; 95(5):869-77. DOI: 10.1002/jsfa.6789. View

Alrajhi A, Alsahli A, Alhelal I, Rihan H, Fuller M, Alsadon A . The Effect of LED Light Spectra on the Growth, Yield and Nutritional Value of Red and Green Lettuce (). Plants (Basel). 2023; 12(3). PMC: 9919669. DOI: 10.3390/plants12030463. View

Sathasivam R, Park S, Kim J, Park Y, Kim M, Nguyen B . Metabolic Profiling of Primary and Secondary Metabolites in Kohlrabi ( var. ) Sprouts Exposed to Different Light-Emitting Diodes. Plants (Basel). 2023; 12(6). PMC: 10057582. DOI: 10.3390/plants12061296. View

Trivellini A, Toscano S, Romano D, Ferrante A . The Role of Blue and Red Light in the Orchestration of Secondary Metabolites, Nutrient Transport and Plant Quality. Plants (Basel). 2023; 12(10). PMC: 10223693. DOI: 10.3390/plants12102026. View

Wang X, Wang Q, Nguyen P, Lin C . Cryptochrome-mediated light responses in plants. Enzymes. 2015; 35:167-89. PMC: 6167252. DOI: 10.1016/B978-0-12-801922-1.00007-5. View

Kwon Y, Lee J, Roh Y, Choi I, Kim Y, Kim J . Effect of Supplemental Inter-Lighting on Paprika Cultivated in an Unheated Greenhouse in Summer Using Various Light-Emitting Diodes. Plants (Basel). 2023; 12(8). PMC: 10143467. DOI: 10.3390/plants12081684. View

Sobczak A, Sujkowska-Rybkowska M, Gajc-Wolska J, Kowalczyk W, Borucki W, Kalaji H . Photosynthetic Efficiency and Anatomical Structure of Pepper Leaf ( L.) Transplants Grown under High-Pressure Sodium (HPS) and Light-Emitting Diode (LED) Supplementary Lighting Systems. Plants (Basel). 2021; 10(10). PMC: 8541379. DOI: 10.3390/plants10101975. View

10.

Ma L, Li J, Qu L, Hager J, Chen Z, Zhao H . Light control of Arabidopsis development entails coordinated regulation of genome expression and cellular pathways. Plant Cell. 2001; 13(12):2589-607. PMC: 139475. DOI: 10.1105/tpc.010229. View

11.

Ouzounis T, Parjikolaei B, Frette X, Rosenqvist E, Ottosen C . Predawn and high intensity application of supplemental blue light decreases the quantum yield of PSII and enhances the amount of phenolic acids, flavonoids, and pigments in Lactuca sativa. Front Plant Sci. 2015; 6:19. PMC: 4341431. DOI: 10.3389/fpls.2015.00019. View

12.

Untergasser A, Ruijter J, Benes V, van den Hoff M . Web-based LinRegPCR: application for the visualization and analysis of (RT)-qPCR amplification and melting data. BMC Bioinformatics. 2021; 22(1):398. PMC: 8386043. DOI: 10.1186/s12859-021-04306-1. View

13.

James S, Smith W, Vogelmann T . Ontogenetic differences in mesophyll structure and chlorophyll distribution in Eucalyptus globulus ssp. globulus. Am J Bot. 2011; 86(2):198-207. View

14.

Prioul J, Brangeon J, Reyss A . Interaction between External and Internal Conditions in the Development of Photosynthetic Features in a Grass Leaf: I. REGIONAL RESPONSES ALONG A LEAF DURING AND AFTER LOW-LIGHT OR HIGH-LIGHT ACCLIMATION. Plant Physiol. 1980; 66(4):762-9. PMC: 440719. DOI: 10.1104/pp.66.4.762. View

15.

Wang S, Fang H, Xie J, Wu Y, Tang Z, Liu Z . Physiological Responses of Cucumber Seedlings to Different Supplemental Light Duration of Red and Blue LED. Front Plant Sci. 2021; 12:709313. PMC: 8311605. DOI: 10.3389/fpls.2021.709313. View

16.

Casal J, Candia A, Sellaro R . Light perception and signalling by phytochrome A. J Exp Bot. 2013; 65(11):2835-45. DOI: 10.1093/jxb/ert379. View

17.

Becker C, Klaring H, Kroh L, Krumbein A . Temporary reduction of radiation does not permanently reduce flavonoid glycosides and phenolic acids in red lettuce. Plant Physiol Biochem. 2013; 72:154-60. DOI: 10.1016/j.plaphy.2013.05.006. View

18.

Rana A, Samtiya M, Dhewa T, Mishra V, Aluko R . Health benefits of polyphenols: A concise review. J Food Biochem. 2022; 46(10):e14264. DOI: 10.1111/jfbc.14264. View

19.

Pu G, Wang P, Zhou B, Liu Z, Xiang F . Cloning and characterization of Lonicera japonica p-coumaroyl ester 3-hydroxylase which is involved in the biosynthesis of chlorogenic acid. Biosci Biotechnol Biochem. 2013; 77(7):1403-9. DOI: 10.1271/bbb.130011. View

20.

Wang J, Lu W, Tong Y, Yang Q . Leaf Morphology, Photosynthetic Performance, Chlorophyll Fluorescence, Stomatal Development of Lettuce (Lactuca sativa L.) Exposed to Different Ratios of Red Light to Blue Light. Front Plant Sci. 2016; 7:250. PMC: 4785143. DOI: 10.3389/fpls.2016.00250. View