Associations Between Various Inflammatory Markers and Carotid Findings in a Voluntary Asymptomatic Population Sample

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Sep 14

PMID 39273602

Authors

Balazs Bence Nyarady

Edit Dosa

Laszlo Kohidai

Eva Pallinger

Renata Guban

Adam Szonyi

Loretta Zsuzsa Kiss

Zsolt Bagyura

Affiliations

Soon will be listed here.

Abstract

Cardiovascular disease (CVD) is the leading cause of morbidity and mortality worldwide, and atherosclerosis is the key factor promoting its development. Carotid intima-media thickening and the presence of carotid plaques are important indices of cardiovascular risk. In addition, inflammation is a major and complex factor in the development of atherosclerosis. The relationships between carotid atherosclerosis and certain inflammatory markers have rarely been studied in healthy individuals. Therefore, we aimed to investigate the associations between subclinical carotid atherosclerosis and various inflammatory biomarkers in a large Caucasian population free of evident CVD. In addition to recording study participants' demographic characteristics, anthropometric characteristics, and atherosclerotic risk factors, laboratory tests were performed to measure levels of hemoglobin A1c (HbA1c), high-sensitivity C-reactive protein, and inflammatory cytokines/chemokines, including interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-18, IL-23, IL-33, interferon (IFN)-α2, IFN-γ, tumor necrosis factor-α, and monocyte chemoattractant protein (MCP)-1. This study included 264 asymptomatic individuals with a median age of 61.7 years (interquartile range, 54.5-67.5 years); 45.7% of participants were male. Participants were divided into two groups according to their carotid status: the normal carotid group, comprising 120 participants; and the pathological carotid group, comprising 144 participants. Compared with the normal carotid group, hypertension and diabetes mellitus were significantly more common and serum levels of HbA1c, IL-8, and MCP-1 were significantly higher in the pathological carotid group. Multivariate regression analysis revealed significant positive associations between pathological carotid findings and serum levels of IL-8 (highest tertile, OR: 2.4, = 0.030) and MCP-1 (highest tertile, OR: 2.4, = 0.040). Our results suggest that IL-8 and MCP-1 may serve as early indicators of subclinical atherosclerosis, thereby helping to identify individuals at increased risk of CVD before the onset of clinical symptoms.

References

Touboul P, Hennerici M, Meairs S, Adams H, Amarenco P, Bornstein N . Mannheim carotid intima-media thickness and plaque consensus (2004-2006-2011). An update on behalf of the advisory board of the 3rd, 4th and 5th watching the risk symposia, at the 13th, 15th and 20th European Stroke Conferences, Mannheim, Germany,.... Cerebrovasc Dis. 2012; 34(4):290-6. PMC: 3760791. DOI: 10.1159/000343145. View

Taleb S, Tedgui A, Mallat Z . IL-17 and Th17 cells in atherosclerosis: subtle and contextual roles. Arterioscler Thromb Vasc Biol. 2014; 35(2):258-64. DOI: 10.1161/ATVBAHA.114.303567. View

Susser L, Rayner K . Through the layers: how macrophages drive atherosclerosis across the vessel wall. J Clin Invest. 2022; 132(9). PMC: 9057606. DOI: 10.1172/JCI157011. View

Soni M, Ambrosino M, Jacoby D . The Use of Subclinical Atherosclerosis Imaging to Guide Preventive Cardiology Management. Curr Cardiol Rep. 2021; 23(6):61. DOI: 10.1007/s11886-021-01490-7. View

Azurmendi P, Fraga A, Galan F, Kotliar C, Arrizurieta E, Valdez M . Early renal and vascular changes in ADPKD patients with low-grade albumin excretion and normal renal function. Nephrol Dial Transplant. 2009; 24(8):2458-63. DOI: 10.1093/ndt/gfp136. View

Basurto L, Gregory M, Hernandez S, Sanchez-Huerta L, Martinez A, Manuel-Apolinar L . Monocyte chemoattractant protein-1 (MCP-1) and fibroblast growth factor-21 (FGF-21) as biomarkers of subclinical atherosclerosis in women. Exp Gerontol. 2019; 124:110624. DOI: 10.1016/j.exger.2019.05.013. View

Isoda K, Sawada S, Ishigami N, Matsuki T, Miyazaki K, Kusuhara M . Lack of interleukin-1 receptor antagonist modulates plaque composition in apolipoprotein E-deficient mice. Arterioscler Thromb Vasc Biol. 2004; 24(6):1068-73. DOI: 10.1161/01.ATV.0000127025.48140.a3. View

. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA. 2013; 310(20):2191-4. DOI: 10.1001/jama.2013.281053. View

Bjorkegren J, Lusis A . Atherosclerosis: Recent developments. Cell. 2022; 185(10):1630-1645. PMC: 9119695. DOI: 10.1016/j.cell.2022.04.004. View

10.

Libby P, Ridker P, Hansson G . Progress and challenges in translating the biology of atherosclerosis. Nature. 2011; 473(7347):317-25. DOI: 10.1038/nature10146. View

11.

Lee W, Allison M, Kim D, Song C, Barrett-Connor E . Association of interleukin-6 and C-reactive protein with subclinical carotid atherosclerosis (the Rancho Bernardo Study). Am J Cardiol. 2007; 99(1):99-102. DOI: 10.1016/j.amjcard.2006.07.070. View

12.

Bagyura Z, Kiss L, Edes E, Lux A, Polgar L, Soos P . [Cardiovascular screening programme in the Central Hungarian region. The Budakalász Study]. Orv Hetil. 2014; 155(34):1344-52. DOI: 10.1556/OH.2014.29969. View

13.

von Ehr A, Bode C, Hilgendorf I . Macrophages in Atheromatous Plaque Developmental Stages. Front Cardiovasc Med. 2022; 9:865367. PMC: 9081876. DOI: 10.3389/fcvm.2022.865367. View

14.

Goossens P, Gijbels M, Zernecke A, Eijgelaar W, Vergouwe M, van der Made I . Myeloid type I interferon signaling promotes atherosclerosis by stimulating macrophage recruitment to lesions. Cell Metab. 2010; 12(2):142-53. DOI: 10.1016/j.cmet.2010.06.008. View

15.

Miller A, Xu D, Asquith D, Denby L, Li Y, Sattar N . IL-33 reduces the development of atherosclerosis. J Exp Med. 2008; 205(2):339-46. PMC: 2271006. DOI: 10.1084/jem.20071868. View

16.

Parra S, Coll B, Aragones G, Marsillach J, Beltran R, Rull A . Nonconcordance between subclinical atherosclerosis and the calculated Framingham risk score in HIV-infected patients: relationships with serum markers of oxidation and inflammation. HIV Med. 2009; 11(4):225-31. DOI: 10.1111/j.1468-1293.2009.00766.x. View

17.

Hofstedt O, Wahlin B, Sodergren A . Associations between serological biomarkers and subclinical atherosclerosis in patients with rheumatoid arthritis after 11 years of follow-up. Clin Exp Rheumatol. 2023; 42(5):967-973. DOI: 10.55563/clinexprheumatol/70qiy8. View

18.

Kolliker Frers R, Cosentino V, Tau J, Kerzberg E, Urdapilleta A, Chiocconi M . Immune-Mediated Inflammation Promotes Subclinical Atherosclerosis in Recent-Onset Psoriatic Arthritis Patients without Conventional Cardiovascular Risk Factors. Front Immunol. 2018; 9:139. PMC: 5834432. DOI: 10.3389/fimmu.2018.00139. View

19.

Moreno Velasquez I, Malarstig A, Baldassarre D, Borne Y, de Faire U, Engstrom G . Causal analysis of plasma IL-8 on carotid intima media thickness, a measure of subclinical atherosclerosis. Curr Res Transl Med. 2022; 71(1):103374. DOI: 10.1016/j.retram.2022.103374. View

20.

Kirii H, Niwa T, Yamada Y, Wada H, Saito K, Iwakura Y . Lack of interleukin-1beta decreases the severity of atherosclerosis in ApoE-deficient mice. Arterioscler Thromb Vasc Biol. 2003; 23(4):656-60. DOI: 10.1161/01.ATV.0000064374.15232.C3. View