Using Transcriptomics to Determine the Mechanism for the Resistance to Fusarium Head Blight of a Wheat- Translocation Line

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Sep 14

PMID 39273397

Authors

Yi Dai

Wenlin Fei

Shiqiang Chen

Juntao Shi

Haigang Ma

Haifeng Li

Jinfeng Li

Yonggang Wang

Yujiao Gao

Jinghuan Zhu

Bingkui Wang

Jianmin Chen

Hongxiang Ma

Affiliations

Soon will be listed here.

Abstract

Fusarium head blight (FHB), caused by the species complex, is a destructive disease in wheat worldwide. The lack of FHB-resistant germplasm is a barrier in wheat breeding for resistance to FHB. is an important relative that has been successfully used for the genetic improvement of wheat. In this study, a translocation line, YNM158, with the YM158 genetic background carrying a fragment of diploid 7EL chromosome created using Co-γ radiation, showed high resistance to FHB under both field and greenhouse conditions. Transcriptome analysis confirmed that the horizontal transfer gene, encoding glutathione S-transferase (), is an important contributor to FHB resistance in the pathogen infection stage, whereas the 7EL chromosome fragment carries other genes regulated by during the colonization stage. Introgression of the 7EL fragment affected the expression of wheat genes that were enriched in resistance pathways, including the phosphatidylinositol signaling system, protein processing in the endoplasmic reticulum, plant-pathogen interaction, and the mitogen-activated protein kinase (MAPK) signaling pathway at different stages after infection. This study provides a novel germplasm for wheat resistance to FHB and new insights into the molecular mechanisms of wheat resistance to FHB.

References

Gonorazky G, Guzzo M, Abd-El-Haliem A, Joosten M, Laxalt A . Silencing of the tomato phosphatidylinositol-phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea. Mol Plant Pathol. 2016; 17(9):1354-1363. PMC: 6638316. DOI: 10.1111/mpp.12365. View

Cainong J, Bockus W, Feng Y, Chen P, Qi L, Sehgal S . Chromosome engineering, mapping, and transferring of resistance to Fusarium head blight disease from Elymus tsukushiensis into wheat. Theor Appl Genet. 2015; 128(6):1019-27. DOI: 10.1007/s00122-015-2485-1. View

Edwards R, Dixon D, Walbot V . Plant glutathione S-transferases: enzymes with multiple functions in sickness and in health. Trends Plant Sci. 2000; 5(5):193-8. DOI: 10.1016/s1360-1385(00)01601-0. View

Kim N, Hwang B . Pepper heat shock protein 70a interacts with the type III effector AvrBsT and triggers plant cell death and immunity. Plant Physiol. 2014; 167(2):307-22. PMC: 4326749. DOI: 10.1104/pp.114.253898. View

Guo J, Zhang X, Hou Y, Cai J, Shen X, Zhou T . High-density mapping of the major FHB resistance gene Fhb7 derived from Thinopyrum ponticum and its pyramiding with Fhb1 by marker-assisted selection. Theor Appl Genet. 2015; 128(11):2301-16. DOI: 10.1007/s00122-015-2586-x. View

Gong B, Zhu W, Li S, Wang Y, Xu L, Wang Y . Molecular cytogenetic characterization of wheat-Elymus repens chromosomal translocation lines with resistance to Fusarium head blight and stripe rust. BMC Plant Biol. 2019; 19(1):590. PMC: 6935081. DOI: 10.1186/s12870-019-2208-x. View

Gong H, Jiao Y, Hu W, Pua E . Expression of glutathione-S-transferase and its role in plant growth and development in vivo and shoot morphogenesis in vitro. Plant Mol Biol. 2005; 57(1):53-66. DOI: 10.1007/s11103-004-4516-1. View

Song R, Cheng Y, Wen M, Song X, Wang T, Xia M . Transferring a new Fusarium head blight resistance locus FhbRc1 from Roegneria ciliaris into wheat by developing alien translocation lines. Theor Appl Genet. 2023; 136(3):36. DOI: 10.1007/s00122-023-04278-1. View

Xue S, Xu F, Tang M, Zhou Y, Li G, An X . Precise mapping Fhb5, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor Appl Genet. 2011; 123(6):1055-63. DOI: 10.1007/s00122-011-1647-z. View

10.

Adachi H, Yoshioka H . Kinase-mediated orchestration of NADPH oxidase in plant immunity. Brief Funct Genomics. 2015; 14(4):253-9. DOI: 10.1093/bfgp/elv004. View

11.

Cuthbert P, Somers D, Brule-Babel A . Mapping of Fhb2 on chromosome 6BS: a gene controlling Fusarium head blight field resistance in bread wheat (Triticum aestivum L.). Theor Appl Genet. 2006; 114(3):429-37. DOI: 10.1007/s00122-006-0439-3. View

12.

Torres M, Dangl J, Jones J . Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response. Proc Natl Acad Sci U S A. 2002; 99(1):517-22. PMC: 117592. DOI: 10.1073/pnas.012452499. View

13.

Harewood L, Fraser P . The impact of chromosomal rearrangements on regulation of gene expression. Hum Mol Genet. 2014; 23(R1):R76-82. DOI: 10.1093/hmg/ddu278. View

14.

Asai S, Ohta K, Yoshioka H . MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana. Plant Cell. 2008; 20(5):1390-406. PMC: 2438462. DOI: 10.1105/tpc.107.055855. View

15.

Dean J, Goodwin P, Hsiang T . Induction of glutathione S-transferase genes of Nicotiana benthamiana following infection by Colletotrichum destructivum and C. orbiculare and involvement of one in resistance. J Exp Bot. 2005; 56(416):1525-33. DOI: 10.1093/jxb/eri145. View

16.

Zhang W, Danilova T, Zhang M, Ren S, Zhu X, Zhang Q . Cytogenetic and genomic characterization of a novel tall wheatgrass-derived Fhb7 allele integrated into wheat B genome. Theor Appl Genet. 2022; 135(12):4409-4419. DOI: 10.1007/s00122-022-04228-3. View

17.

Yang X, Xu M, Wang Y, Cheng X, Huang C, Zhang H . Development and Molecular Cytogenetic Identification of Two Wheat- Roth 7M Chromosome Substitution Lines with Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust. Int J Mol Sci. 2022; 23(13). PMC: 9266563. DOI: 10.3390/ijms23137056. View

18.

Qi L, Pumphrey M, Friebe B, Chen P, Gill B . Molecular cytogenetic characterization of alien introgressions with gene Fhb3 for resistance to Fusarium head blight disease of wheat. Theor Appl Genet. 2008; 117(7):1155-66. DOI: 10.1007/s00122-008-0853-9. View

19.

Coleman A, Maroschek J, Raasch L, Takken F, Ranf S, Huckelhoven R . The Arabidopsis leucine-rich repeat receptor-like kinase MIK2 is a crucial component of early immune responses to a fungal-derived elicitor. New Phytol. 2020; 229(6):3453-3466. DOI: 10.1111/nph.17122. View

20.

Xue S, Li G, Jia H, Xu F, Lin F, Tang M . Fine mapping Fhb4, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor Appl Genet. 2010; 121(1):147-56. DOI: 10.1007/s00122-010-1298-5. View