A Mutant from Escherichia Coli Which Lacks Ribosomal Proteins S17 and L29 Used to Localize These Two Proteins on the Ribosomal Surface

Overview

Journal Eur J Biochem

Specialty Biochemistry

Date 1985 Aug 1

PMID 3926498

Citations 7

Authors

M Stoffler-Meilicke

E R Dabbs

G Stoffler

Affiliations

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Abstract

A mutant of Escherichia coli has been isolated which lacked ribosomal proteins S17 and L29, as judged by two-dimensional gel electrophoresis. A battery of immunological tests was used to confirm this result. Ribosomes of this mutant were used as a control for the localization of proteins S17 and L29 on the surface of the ribosomal subunits of E. coli. Protein S17 has been localized on the 30S subunit body, 3-5 nm away from the lower pole, while protein L29 is located at the back of the 50S particle on the opposite side to the interface.

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