TRIM59 is Required for Mouse GC-1 cell Maintenance Through Modulating the Ubiquitination of AXIN1

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Sep 12

PMID 39263074

Authors

Tiantian Wu

Hui Zhou

Lulu Wang

Jianxin Tan

Wenxin Gao

Yibo Wu

Dan Zhao

Cong Shen

Bo Zheng

Xiaoyan Huang

Binbin Shao

Affiliations

Soon will be listed here.

Abstract

Tripartite motif-containing protein 59 (TRIM59) is a biomarker for multiple tumors with crucial roles. However, the specific role of TRIM59 in germ cells remains largely unknown. Here, we investigated the effects and underlying regulatory mechanisms of TRIM59 on germ cells using the mouse spermatogonial cell line GC-1. Our results demonstrated that TRIM59 promoted proliferation and inhibited apoptosis of GC-1 cells. Mechanistically, TRIM59 maintained GC-1 cell behaviors through ubiquitination of AXIN1 to activate β-catenin signaling. Furthermore, activation of β-catenin signaling reversed the effects mediated by knockdown in GC-1 cells. Collectively, our study revealed a major role and regulatory mechanism of TRIM59 in GC-1 cells, which sheds new light on the molecular pathogenesis of defects in spermatogenesis and may provide therapeutic targets for treatment of male infertility.

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