Endomembrane Trafficking Driven by Microtubule Growth Regulates Stomatal Movement in Arabidopsis

Overview

Journal Nat Commun

Specialty Biology

Date 2024 Sep 11

PMID 39261498

Authors

Hua Zhong

Shuwei Wang

Yaohui Huang

Xiankui Cui

Xuening Ding

Lei Zhu

Ming Yuan

Ying Fu

Affiliations

Soon will be listed here.

Abstract

Microtubule-based vesicle trafficking usually relies upon kinesin and dynein motors and few reports describe microtubule polymerisation driving directional vesicle trafficking. Here we show that Arabidopsis END BINDING1b (EB1b), a microtubule plus-end binding protein, directly interacts with SYP121, a SNARE protein that mediates the trafficking of the K channel KAT1 and its distribution to the plasma membrane (PM) in Arabidopsis guard cells. Knockout of AtEB1b and its homologous proteins results in a modest but significant change in the distribution of KAT1 and SYP121 in guard cells and consequently delays light-induced stomatal opening. Live-cell imaging reveals that a portion of SYP121-associated endomembrane compartments co-localise with AtEB1b at the growing ends of microtubules, trafficking along with the growth of microtubules for targeting to the PM. Our study reveals a mechanism of vesicle trafficking driven by microtubule growth, which is involved in the redistribution of PM proteins to modulate guard cell movement.

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