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A Specific and Ultrasensitive Cas12a/crRNA Assay with Recombinase Polymerase Amplification and Lateral Flow Biosensor Technology for the Rapid Detection of

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Specialty Microbiology
Date 2024 Sep 10
PMID 39254031
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Abstract

Importance: Patients may experience a range of symptoms due to infections, including superficial skin infections, pharyngitis, and invasive diseases in subcutaneous tissues like streptococcal toxic shock syndrome. At present, the clinical diagnostic detection of is based on serological identification, culture, and PCR. These detection methods are time-consuming and require sophisticated equipment, making these methods challenging for routine laboratories. Thus, there is a need for a detection platform that is capable of quickly and accurately identifying . In this study, a rapid and sensitive Cas12a/crRNA assay using recombinase RPA and LFB was developed to detect . The Cas12a/crRNA-based assay exhibited high specificity among different bacteria strains and extremely high sensitivity. This method probably plays an important role for detection and screening.

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