Physiological and Gene Expression Responses of Protohermes Xanthodes (Megaloptera: Corydalidae) Larvae to Imidacloprid

Overview

Journal Naturwissenschaften

Specialty Science

Date 2024 Sep 9

PMID 39249498

Authors

Mao-Zhou Xu

Yu-Tong Li

Cheng-Quan Cao

Affiliations

Soon will be listed here.

Abstract

Megaloptera larvae are important bioindicator species and potential resource insects. To further cultivate their economic role, their living environment must be examined in more detail. In this study, we analyzed the physiological and biochemical effects of a sublethal dose of imidacloprid, a widely used neonicotinoid insecticide, on the larvae of Protohermes xanthodes. After treatment with imidacloprid, P. xanthodes larvae exhibited clear symptoms of poisoning, including the head curling up toward the ventral surface. Additionally, the activity of acetylcholinesterase was significantly inhibited following exposure. The activities of glutathione S-transferases initially continuously increased but showed a slight decrease after 8 days. Catalase activity initially increased and then decreased following imidacloprid treatment; superoxide dismutase activity fluctuated over time, and peroxidase activity continuously increased. The expression levels of HSP70s genes were evaluated using qRT-PCR. These results indicate that P. xanthodes larvae exhibit a toxic response to imidacloprid exposure, manifested as oxidative stress, as observed through behavioral and physiological indicators.

References

Adak T, Kumar J, Shakil N, Walia S . Development of controlled release formulations of imidacloprid employing novel nano-ranged amphiphilic polymers. J Environ Sci Health B. 2012; 47(3):217-25. DOI: 10.1080/03601234.2012.634365. View

Ahmad M, Syma S, Mahmood R . Cr(VI) induces lipid peroxidation, protein oxidation and alters the activities of antioxidant enzymes in human erythrocytes. Biol Trace Elem Res. 2011; 144(1-3):426-35. DOI: 10.1007/s12011-011-9119-5. View

Batista-Silva W, Heinemann B, Rugen N, Nunes-Nesi A, Araujo W, Braun H . The role of amino acid metabolism during abiotic stress release. Plant Cell Environ. 2019; 42(5):1630-1644. DOI: 10.1111/pce.13518. View

Bernabo P, Gaglio M, Bellamoli F, Viero G, Lencioni V . DNA damage and translational response during detoxification from copper exposure in a wild population of Chironomus riparius. Chemosphere. 2017; 173:235-244. DOI: 10.1016/j.chemosphere.2017.01.052. View

Bhattacharyya J, DATTA A . Studies on the effects of lipopolysaccharide on lipid peroxidation of erythrocyte and its reversal by mannitol and glycerol. J Physiol Pharmacol. 2001; 52(1):145-52. View

Bustin S, Benes V, Garson J, Hellemans J, Huggett J, Kubista M . The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009; 55(4):611-22. DOI: 10.1373/clinchem.2008.112797. View

Cartereau A, Taillebois E, Le Questel J, Thany S . Mode of Action of Neonicotinoid Insecticides Imidacloprid and Thiacloprid to the Cockroach Pameα7 Nicotinic Acetylcholine Receptor. Int J Mol Sci. 2021; 22(18). PMC: 8471617. DOI: 10.3390/ijms22189880. View

Cavallaro M, Morrissey C, Headley J, Peru K, Liber K . Comparative chronic toxicity of imidacloprid, clothianidin, and thiamethoxam to Chironomus dilutus and estimation of toxic equivalency factors. Environ Toxicol Chem. 2016; 36(2):372-382. DOI: 10.1002/etc.3536. View

Chen C, Hu W, Zhang R, Jiang A, Liu C . Effects of hydrogen sulfide on the surface whitening and physiological responses of fresh-cut carrots. J Sci Food Agric. 2018; 98(12):4726-4732. DOI: 10.1002/jsfa.9007. View

10.

Cheung C, Zheng G, Li A, Richardson B, Lam P . Relationships between tissue concentrations of polycyclic aromatic hydrocarbons and antioxidative responses of marine mussels, Perna viridis. Aquat Toxicol. 2001; 52(3-4):189-203. DOI: 10.1016/s0166-445x(00)00145-4. View

11.

Cloyd R, Bethke J . Impact of neonicotinoid insecticides on natural enemies in greenhouse and interiorscape environments. Pest Manag Sci. 2010; 67(1):3-9. DOI: 10.1002/ps.2015. View

12.

Dampc J, Kula-Maximenko M, Molon M, Durak R . Enzymatic Defense Response of Apple Aphid to Increased Temperature. Insects. 2020; 11(7). PMC: 7411948. DOI: 10.3390/insects11070436. View

13.

Docile T, Figueiro R, Portela C, Nessimian J . Macroinvertebrate diversity loss in urban streams from tropical forests. Environ Monit Assess. 2016; 188(4):237. DOI: 10.1007/s10661-016-5237-z. View

14.

Dumas P, Morin M, Boquel S, Moffat C, Morin P . Expression status of heat shock proteins in response to cold, heat, or insecticide exposure in the Colorado potato beetle Leptinotarsa decemlineata. Cell Stress Chaperones. 2019; 24(3):539-547. PMC: 6527667. DOI: 10.1007/s12192-019-00983-3. View

15.

Enayati A, Ranson H, Hemingway J . Insect glutathione transferases and insecticide resistance. Insect Mol Biol. 2005; 14(1):3-8. DOI: 10.1111/j.1365-2583.2004.00529.x. View

16.

Fan W, Li G, Zhang X, Wang Y, Wang C, Xu B . The role of melatonin and Tryptophan-5-hydroxylase-1 in different abiotic stressors in Apis cerana cerana. J Insect Physiol. 2020; 128:104180. DOI: 10.1016/j.jinsphys.2020.104180. View

17.

Felton G, Summers C . Antioxidant systems in insects. Arch Insect Biochem Physiol. 1995; 29(2):187-97. DOI: 10.1002/arch.940290208. View

18.

Flores-Cespedes F, Figueredo-Flores C, Daza-Fernandez I, Vidal-Pena F, Villafranca-Sanchez M, Fernandez-Perez M . Preparation and characterization of imidacloprid lignin-polyethylene glycol matrices coated with ethylcellulose. J Agric Food Chem. 2012; 60(4):1042-51. DOI: 10.1021/jf2037483. View

19.

Focks A, Belgers D, Boerwinkel M, Buijse L, Roessink I, van den Brink P . Calibration and validation of toxicokinetic-toxicodynamic models for three neonicotinoids and some aquatic macroinvertebrates. Ecotoxicology. 2018; 27(7):992-1007. PMC: 6132984. DOI: 10.1007/s10646-018-1940-6. View

20.

Frew J, Brown J, Fitzsimmons P, Hoffman A, Sadilek M, Grue C . Toxicokinetics of the neonicotinoid insecticide imidacloprid in rainbow trout (Oncorhynchus mykiss). Comp Biochem Physiol C Toxicol Pharmacol. 2018; 205:34-42. PMC: 5847319. DOI: 10.1016/j.cbpc.2018.01.002. View