Phage-based Biocontrol of Through Indirect Targeting

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2024 Sep 9

PMID 39248462

Authors

Chuncheng Wu

Jumpei Fujiki

Jacques Mathieu

Cory Schwarz

Carolyn Cornell

Pedro J J Alvarez

Affiliations

Soon will be listed here.

Abstract

Importance: Lytic phages are valuable agents for targeted elimination of bacteria in diverse applications. Nevertheless, lytic phages are difficult to isolate for some target pathogens. We offer proof of concept that this limitation may be overcome via indirect phage targeting, which involves knocking out species that interact closely with and benefit the primary problematic target bacteria. Our target () only forms a periodontal pathogenic biofilm if the pioneer colonizer () offers its surface for to attach. Phage predation of the co-adhesive significantly reduced abundance of the target pathogen by >99%, decreased the total biofilm biomass by >44%, and suppressed its production of cytotoxic metabolic byproducts. Thus, this research extends the scope of phage-based biocontrol for public health protection.

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