Microbiological Investigation of Pregnancies Following Vaginal Radical Trachelectomy Using 16S RRNA Sequencing of FFPE Placental Specimens

Overview

Journal FEBS Open Bio

Specialty Biology

Date 2024 Sep 9

PMID 39245884

Authors

Risa Tsunematsu

Tasuku Mariya

Mina Umemoto

Shiori Ogawa

Wataru Arai

Suguru E Tanaka

Kyota Ashikawa

Terufumi Kubo

Yoshiyuki Sakuraba

Tsuyoshi Baba

Shinichi Ishioka

Toshiaki Endo

Tsuyoshi Saito

Affiliations

Soon will be listed here.

Abstract

This study examined the risk of intrauterine infection associated with radical trachelectomy (RT) in early-stage cervical cancer patients. This procedure preserves fertility but is linked to increased risk of intrauterine infection due to cervical defects during pregnancy. DNA was extracted from the formalin-fixed paraffin-embedded (FFPE) placental specimens of 23 pregnant post-RT patients and 16S rRNA gene sequencing was used for bacterial identification. The prevalence of Lactobacillus crispatus and Burkholderia stabilis was significantly higher in the non-chorioamnionitis group. In contrast, alpha diversity analysis using the PD index showed significantly higher diversity in the chorioamnionitis group (P = 0.04). The demonstrated relationship between chorioamnionitis and microbial diversity affirms the importance of controlling the genital bacterial flora in pregnancies following RT.

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