PIN1 is a Novel Interaction Partner and a Negative Upstream Regulator of the Transcription Factor NFIB

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2024 Sep 8

PMID 39245791

Authors

Sinem Saritas Erdogan

Ahmet Erdal Yilmaz

Asli Kumbasar

Affiliations

Soon will be listed here.

Abstract

NFIB is a transcription factor of the Nuclear Factor One (NFI) family that is essential for embryonic development. Post-translational control of NFIB or its upstream regulators have not been well characterized. Here, we show that PIN1 binds NFIB in a phosphorylation-dependent manner, via its WW domain. PIN1 interacts with the well-conserved N-terminal domains of all NFIs. Moreover, PIN1 attenuates the transcriptional activity of NFIB; this attenuation requires substrate binding by PIN1 but not its isomerase activity. Paradoxically, we found stabilization of NFIB by PIN1. We propose that PIN1 represses NFIB function not by regulating its abundance but by inducing a conformational change. These results identify NFIB as a novel PIN1 target and posit a role for PIN1 in post-translational regulation of NFIB and other NFIs.

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