Prebiotic Effects of Commercial Apple Juice in High-fat Diet Fed Rat

Overview

Journal BMC Res Notes

Publisher Biomed Central

Specialties Biology
General Medicine

Date 2024 Sep 5

PMID 39237963

Authors

Risako Kon

Nobutomo Ikarashi

Mayumi Ohkuma

Misato Toyonaga

Rei Tomimoto

Hiroyasu Sakai

Tomoo Hosoe

Junzo Kamei

Affiliations

Soon will be listed here.

Abstract

Objectives: Apples are one of the most frequently consumed fruits and are effective in preventing lifestyle-related and other diseases. However, few studies have been conducted to evaluate health benefits of processed apple products such as juice. In this study, we analyzed the health benefits of consuming apple juice, focusing on changes in the gut microbiota, which plays an important role in maintaining human health.

Results: Rats were fed apple juice ad libitum, and the relative abundances of various gut microbiota in fecal samples were analyzed. In addition, rats treated apple juice were fed with a high-fat diet, and body weight, plasma triglyceride, glucose, and cholesterol levels were measured. The relative abundance of Clostridium cluster XIV did not change with the treatment of apple juice, but the relative abundance of Clostridium cluster IV was significantly decreased. In contrast, the relative abundances of Lactobacillus and Bifidobacterium, which provide benefits to the human body, were significantly increased by 3-fold and 10-fold, respectively, with apple juice consumption. When apple juice-treated rats were fed a high-fat diet, the increase in body weight, liver fat, and blood lipid parameters were all suppressed compared to high-fat alone group.

Conculusion: This study suggests that the consumption of apple juice changes the gut microbiota, exerts a prebiotic effect, and is effective in improving lifestyle-related diseases.

References

Maier L, Pruteanu M, Kuhn M, Zeller G, Telzerow A, Anderson E . Extensive impact of non-antibiotic drugs on human gut bacteria. Nature. 2018; 555(7698):623-628. PMC: 6108420. DOI: 10.1038/nature25979. View

Nagasako-Akazome Y, Kanda T, Ohtake Y, Shimasaki H, Kobayashi T . Apple polyphenols influence cholesterol metabolism in healthy subjects with relatively high body mass index. J Oleo Sci. 2007; 56(8):417-28. DOI: 10.5650/jos.56.417. View

Mohammadi T, Pietersz R, Vandenbroucke-Grauls C, Savelkoul P, Reesink H . Detection of bacteria in platelet concentrates: comparison of broad-range real-time 16S rDNA polymerase chain reaction and automated culturing. Transfusion. 2005; 45(5):731-6. DOI: 10.1111/j.1537-2995.2005.04258.x. View

Steenbergen L, Sellaro R, van Hemert S, Bosch J, Colzato L . A randomized controlled trial to test the effect of multispecies probiotics on cognitive reactivity to sad mood. Brain Behav Immun. 2015; 48:258-64. DOI: 10.1016/j.bbi.2015.04.003. View

Hrdy J, Couturier-Maillard A, Boutillier D, Lapadatescu C, Blanc P, Prochazka J . Oral supplementation with selected Lactobacillus acidophilus triggers IL-17-dependent innate defense response, activation of innate lymphoid cells type 3 and improves colitis. Sci Rep. 2022; 12(1):17591. PMC: 9585059. DOI: 10.1038/s41598-022-21643-0. View

Papizadeh M, Rohani M, Nahrevanian H, Javadi A, Pourshafie M . Probiotic characters of Bifidobacterium and Lactobacillus are a result of the ongoing gene acquisition and genome minimization evolutionary trends. Microb Pathog. 2017; 111:118-131. DOI: 10.1016/j.micpath.2017.08.021. View

Sartor R . Microbial influences in inflammatory bowel diseases. Gastroenterology. 2008; 134(2):577-94. DOI: 10.1053/j.gastro.2007.11.059. View

Aron-Wisnewsky J, Warmbrunn M, Nieuwdorp M, Clement K . Metabolism and Metabolic Disorders and the Microbiome: The Intestinal Microbiota Associated With Obesity, Lipid Metabolism, and Metabolic Health-Pathophysiology and Therapeutic Strategies. Gastroenterology. 2020; 160(2):573-599. DOI: 10.1053/j.gastro.2020.10.057. View

Respondek F, Gerard P, Bossis M, Boschat L, Bruneau A, Rabot S . Short-chain fructo-oligosaccharides modulate intestinal microbiota and metabolic parameters of humanized gnotobiotic diet induced obesity mice. PLoS One. 2013; 8(8):e71026. PMC: 3741321. DOI: 10.1371/journal.pone.0071026. View

10.

Reddel S, Del Chierico F, Quagliariello A, Giancristoforo S, Vernocchi P, Russo A . Gut microbiota profile in children affected by atopic dermatitis and evaluation of intestinal persistence of a probiotic mixture. Sci Rep. 2019; 9(1):4996. PMC: 6428866. DOI: 10.1038/s41598-019-41149-6. View

11.

Wu T, Sun M, Liu R, Sui W, Zhang J, Yin J . subsp. Remodeled and Phosphatidylserine Levels and Ameliorated Intestinal Disorders and liver Metabolic Abnormalities Induced by High-Fat Diet. J Agric Food Chem. 2020; 68(16):4632-4640. DOI: 10.1021/acs.jafc.0c00717. View

12.

Wang X, Liu F, Cui Y, Yin Y, Li S, Li X . Apple Polyphenols Extracts Ameliorate High Carbohydrate Diet-Induced Body Weight Gain by Regulating the Gut Microbiota and Appetite. J Agric Food Chem. 2021; 70(1):196-210. DOI: 10.1021/acs.jafc.1c07258. View

13.

Song Y, Kato N, Liu C, Matsumiya Y, Kato H, Watanabe K . Rapid identification of 11 human intestinal Lactobacillus species by multiplex PCR assays using group- and species-specific primers derived from the 16S-23S rRNA intergenic spacer region and its flanking 23S rRNA. FEMS Microbiol Lett. 2000; 187(2):167-73. DOI: 10.1111/j.1574-6968.2000.tb09155.x. View

14.

Song W, Song C, Li L, Wang T, Hu J, Zhu L . Lactobacillus alleviated obesity induced by high-fat diet in mice. J Food Sci. 2021; 86(12):5439-5451. DOI: 10.1111/1750-3841.15971. View

15.

Niwa T, Nakao M, Hoshi S, Yamada K, Inagaki K, Nishida M . Effect of dietary fiber on morphine-induced constipation in rats. Biosci Biotechnol Biochem. 2002; 66(6):1233-40. DOI: 10.1271/bbb.66.1233. View

16.

Song Y, Liu C, Finegold S . Real-time PCR quantitation of clostridia in feces of autistic children. Appl Environ Microbiol. 2004; 70(11):6459-65. PMC: 525120. DOI: 10.1128/AEM.70.11.6459-6465.2004. View

17.

Ogura K, Ogura M, Shoji T, Sato Y, Tahara Y, Yamano G . Oral Administration of Apple Procyanidins Ameliorates Insulin Resistance via Suppression of Pro-Inflammatory Cytokine Expression in Liver of Diabetic ob/ob Mice. J Agric Food Chem. 2016; 64(46):8857-8865. DOI: 10.1021/acs.jafc.6b03424. View

18.

Ikarashi N, Ogawa S, Hirobe R, Kon R, Kusunoki Y, Yamashita M . Epigallocatechin gallate induces a hepatospecific decrease in the CYP3A expression level by altering intestinal flora. Eur J Pharm Sci. 2017; 100:211-218. DOI: 10.1016/j.ejps.2017.01.022. View

19.

Tajima M, Ikarashi N, Igeta S, Toda T, Ishii M, Tanaka Y . Different diets cause alterations in the enteric environment and trigger changes in the expression of hepatic cytochrome P450 3A, a drug-metabolizing enzyme. Biol Pharm Bull. 2013; 36(4):624-34. DOI: 10.1248/bpb.b12-01005. View

20.

Shoji T, Yamada M, Miura T, Nagashima K, Ogura K, Inagaki N . Chronic administration of apple polyphenols ameliorates hyperglycaemia in high-normal and borderline subjects: A randomised, placebo-controlled trial. Diabetes Res Clin Pract. 2017; 129:43-51. DOI: 10.1016/j.diabres.2017.03.028. View