Effects of Soluble Klotho and Wnt/β-catenin Signaling Pathway in Vascular Calcification in Chronic Kidney Disease Model Rats and the Intervention of Shenyuan Granules

Overview

Journal Ren Fail

Publisher Informa Healthcare

Date 2024 Sep 4

PMID 39230198

Authors

Xinrong Zou

Changjiang Wang

Lan Wang

Shenghua Huang

Danfang Deng

Lamei Lin

Xiaoqin Wang

Affiliations

Soon will be listed here.

Abstract

Objective: This study aimed to investigate the effect of the soluble Klotho (sKlotho)/Wnt/β-catenin signaling pathway on vascular calcification in rat models of chronic kidney disease (CKD) and the intervention effect of Shenyuan granules.

Methods: Rats with 5/6 nephrectomy and high phosphorus feeding were used to establish the vascular calcification model. The rats were given gradient doses of Shenyuan granules aqueous solution and calcitriol solution by gavage for 8 weeks, which were divided into experimental group and positive control group.

Results: The 5/6 nephrectomy combined with high phosphorus feeding induced thoracic aortic calcification in rats. Shenyuan granules intervention increased the serum sKlotho level, inhibited the mRNA and protein expression of Wnt1, β-catenin, and Runx2 in the thoracic aorta, and alleviated thoracic aortic media calcification in rats.

Conclusion: Shenyuan granules may partially regulate the Wnt/β-catenin signaling pathway via serum sKl to interfere with the expression of Runx2, thereby improving vascular calcification in CKD.

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