Ultrastructural Localization of Dipicolinic Acid in Dormant Spores of Bacillus Subtilis by Immunoelectron Microscopy with Colloidal Gold Particles
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The localization of dipicolinic acid in dormant spores of Bacillus subtilis was examined by an immunoelectron microscopy method with colloidal gold-immunoglobulin G complex. The colloidal gold particles were distributed mainly in the core regions of dormant spores and were not observed in those of germinated or autoclaved spores. This result clearly demonstrates that dipicolinic acid is localized in the cores of dormant spores.
Nanomechanical Characterization of Bacillus anthracis Spores by Atomic Force Microscopy.
Li A, Burggraf L, Xing Y Appl Environ Microbiol. 2016; 82(10):2988-2999.
PMID: 26969703 PMC: 4959069. DOI: 10.1128/AEM.00431-16.
Zhang J, Dalal N, Matthews M, Waller L, Saunders C, Fox K J Microbiol Methods. 2007; 70(3):442-51.
PMID: 17628729 PMC: 2084089. DOI: 10.1016/j.mimet.2007.05.019.
MORIYAMA R, Fukuoka H, Miyata S, Kudoh S, Hattori A, Kozuka S J Bacteriol. 1999; 181(8):2373-8.
PMID: 10197998 PMC: 93660. DOI: 10.1128/JB.181.8.2373-2378.1999.
Lundin R, SACKS L Appl Environ Microbiol. 1988; 54(4):923-8.
PMID: 3132103 PMC: 202574. DOI: 10.1128/aem.54.4.923-928.1988.
Crook S, Treml S, Collins M J Bacteriol. 1986; 167(1):89-95.
PMID: 3087967 PMC: 212845. DOI: 10.1128/jb.167.1.89-95.1986.