» Articles » PMID: 3922946

Ultrastructural Localization of Dipicolinic Acid in Dormant Spores of Bacillus Subtilis by Immunoelectron Microscopy with Colloidal Gold Particles

Overview
Journal J Bacteriol
Specialty Microbiology
Date 1985 Jun 1
PMID 3922946
Citations 5
Authors
Affiliations
Soon will be listed here.
Abstract

The localization of dipicolinic acid in dormant spores of Bacillus subtilis was examined by an immunoelectron microscopy method with colloidal gold-immunoglobulin G complex. The colloidal gold particles were distributed mainly in the core regions of dormant spores and were not observed in those of germinated or autoclaved spores. This result clearly demonstrates that dipicolinic acid is localized in the cores of dormant spores.

Citing Articles

Nanomechanical Characterization of Bacillus anthracis Spores by Atomic Force Microscopy.

Li A, Burggraf L, Xing Y Appl Environ Microbiol. 2016; 82(10):2988-2999.

PMID: 26969703 PMC: 4959069. DOI: 10.1128/AEM.00431-16.


Supercritical carbon dioxide and hydrogen peroxide cause mild changes in spore structures associated with high killing rate of Bacillus anthracis.

Zhang J, Dalal N, Matthews M, Waller L, Saunders C, Fox K J Microbiol Methods. 2007; 70(3):442-51.

PMID: 17628729 PMC: 2084089. DOI: 10.1016/j.mimet.2007.05.019.


Expression of a germination-specific amidase, SleB, of Bacilli in the forespore compartment of sporulating cells and its localization on the exterior side of the cortex in dormant spores.

MORIYAMA R, Fukuoka H, Miyata S, Kudoh S, Hattori A, Kozuka S J Bacteriol. 1999; 181(8):2373-8.

PMID: 10197998 PMC: 93660. DOI: 10.1128/JB.181.8.2373-2378.1999.


High-resolution solid-state 13C nuclear magnetic resonance of bacterial spores: identification of the alpha-carbon signal of dipicolinic acid.

Lundin R, SACKS L Appl Environ Microbiol. 1988; 54(4):923-8.

PMID: 3132103 PMC: 202574. DOI: 10.1128/aem.54.4.923-928.1988.


Immunocytochemical ultrastructural analysis of chromatophore membrane formation in Rhodospirillum rubrum.

Crook S, Treml S, Collins M J Bacteriol. 1986; 167(1):89-95.

PMID: 3087967 PMC: 212845. DOI: 10.1128/jb.167.1.89-95.1986.

References
1.
HYATT M, LEVINSON H . Water vapor, aqueous ethyl alcohol, and heat activation of Bacillus megaterium spore germination. J Bacteriol. 1968; 95(6):2090-101. PMC: 315139. DOI: 10.1128/jb.95.6.2090-2101.1968. View

2.
HOLBERT P . An effective method of preparing sections of Bacillus polymyxa sporangia and spores for electron microscopy. J Biophys Biochem Cytol. 1960; 7:373-6. PMC: 2224818. DOI: 10.1083/jcb.7.2.373. View

3.
Pearce S, Fitz-James P . Spore refractility in variants of Bacillus cereus treated with actinomycin D. J Bacteriol. 1971; 107(1):337-44. PMC: 246920. DOI: 10.1128/jb.107.1.337-344.1971. View

4.
Scherrer R, Gerhardt P . Location of calcium within Bacillus spores by electron probe x-ray microanalysis. J Bacteriol. 1972; 112(1):559-68. PMC: 251445. DOI: 10.1128/jb.112.1.559-568.1972. View

5.
Leanz G, Gilvarg C . Dipicolinic acid location in intact spores of Bacillus megaterium. J Bacteriol. 1973; 114(1):455-6. PMC: 251789. DOI: 10.1128/jb.114.1.455-456.1973. View