Protocol for Volume Correlative Light X-ray and Electron Microscopy of Endothelial Cells in Mouse Tissue

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2024 Sep 3

PMID 39226173

Authors

Natalia Reglero-Real

Lorena Perez-Gutierrez

Rebeca S Saleeb

Sussan Nourshargh

Lucy Collinson

Azumi Yoshimura

Affiliations

Soon will be listed here.

Abstract

Correlative light and electron microscopy (CLEM) greatly facilitate capturing the ultrastructure of spatially and/or temporally rare events. Here, we present a protocol for targeting regions of interests (ROIs) in tissue endothelial cells (ECs) using X-ray micro-computed tomography (μCT). We describe steps for ROI targeting guided by vasculature patterns and positions of EC nuclei visualized by light and X-ray microscopy. The protocol is applicable to thin or translucent tissues that contain defined landmarks visible in both light and X-ray microscopy. For complete details on the use and execution of this protocol, please refer to Reglero-Real et al..

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