Transcriptome-associated Metabolomics Reveals the Molecular Mechanism of Flavonoid Biosynthesis in (Osbeck.) Merr Under Abiotic Stress

Overview

Journal Front Plant Sci

Date 2024 Sep 3

PMID 39224850

Authors

Hongyang Gao

Xi Huang

Pengfei Lin

Yuqing Hu

Ziqi Zheng

Quan Yang

Affiliations

Soon will be listed here.

Abstract

The primary pharmacological components of (Osbeck.) Merr. are flavonoids, which have a broad range of pharmacological effects and are important in many applications. However, there have been few reports on the molecular mechanisms underlying flavonoid biosynthesis in the pharmacodynamic constituents of . Flavonoid biosynthesis in pharmacodynamic constituents has, however, been rarely studied. In this study, we investigated how salt stress, 6-BA (6-Benzylaminopurine) treatment, and PEG 6000-simulated drought stress affect flavonoid accumulation in leaves. We integrated metabolomics and transcriptomic analysis to map the secondary metabolism regulatory network of and identify key transcription factors involved in flavonoid biosynthesis. We then constructed overexpression vectors for the transcription factors and used them to transiently infiltrate for functional validation. This experiment confirmed that the transcription factor DsMYB60 promotes the production of total flavonoids in L. leaves. This study lays the foundation for studying flavonoid biosynthesis in at the molecular level. Furthermore, this study contributes novel insights into the molecular mechanisms involved in the biosynthesis of active ingredients in medicinal plants.

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