Exploring the Uncharted Seas: Metabolite Profiling Unleashes the Anticancer Properties of

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Sep 3

PMID 39224332

Authors

Bornita Das

Asharani Prusty

Subhajeet Dutta

Aditi Maulik

Yogita Dahat

Deepak Kumar

Sucheta Tripathy

Affiliations

Soon will be listed here.

Abstract

Marine cyanobacteria offer a rich source of varied natural products with both chemical and biological diversity. () is a filamentous non-heterocystous marine cyanobacterium from Oscillatoriaceae family. In this investigation, we have unveiled bioactive extracts from using two distinct solvent systems, revealing significant anticancer properties. Our assessment of the organic and aqueous extracts (MCE and AE) of demonstrated pronounced antiproliferative and antimetastatic effects. Notably, this study is the first to elucidate the anticancer and anti-metastatic potential of extracts in both 2D and 3D cell culture models. Both MCE and AE induced apoptosis, hindered cell proliferation, invasion, and migration in A549 non-small cell lung cancer cells, accompanied by alterations in cell morphology and cytoskeleton collapse. Moreover, MCE and AE induced spheroid disintegration in A549 cells. Transcriptomics analysis highlighted the significant involvement of Rap1 and p53 signaling pathways in mediating the observed antitumor effects. Mass spectroscopy characterization of these extracts identified 11 compounds, some known for their anticancer potential. HPLC analysis of AE revealed six peaks with UV absorption spectra resembling phycocyanin, a cyanobacterial pigment with well-known anticancer activity. Collectively, these findings underscore the anticancer potential of MCE and AE, containing bioactive metabolites with anticancer and antimetastatic properties.

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