A Semisynthetic Oligomannuronic Acid-Based Glycoconjugate Vaccine Against

Overview

Journal ACS Cent Sci

Specialty Chemistry

Date 2024 Sep 2

PMID 39220693

Authors

Yiyue Zhang

Xiaotong Wang

Youling Liang

Liangliang Zhang

Jiahao Fan

You Yang

Affiliations

Soon will be listed here.

Abstract

is one of the leading causes of nosocomial infections and has become increasingly resistant to multiple antibiotics. However, development of novel classes of antibacterial agents against multidrug-resistant is extremely difficult. Herein we develop a semisynthetic oligomannuronic acid-based glycoconjugate vaccine that confers broad protection against infections of both mucoid and nonmucoid strains of . The well-defined glycoconjugate vaccine formulated with Freund's adjuvant (FA) employing a highly conserved antigen elicited a strong and specific immune response and protected mice against both mucoid and nonmucoid strains of . The resulting antibodies recognized different strains of and mediated the opsonic killing of the bacteria at varied levels depending on the amount of alginate expressed on the surface of the strains. Vaccination with the glycoconjugate vaccine plus FA significantly promoted the pulmonary and blood clearance of the mucoid PAC1 strain of and considerably improved the survival rates of mice against the nonmucoid PAO1 strain of . Thus, the semisynthetic glycoconjugate is a promising vaccine that may provide broad protection against both types of .

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