Biocompatibility and Bone Regeneration with Elastin-like Recombinamer-based Catalyst-free Click Gels

Overview

Journal Sci Rep

Specialty Science

Date 2024 Aug 30

PMID 39215050

Authors

I N Camal Ruggieri

M Aimone

D Juanes-Gusano

A Ibanez-Fonseca

O Santiago

M Stur

J P Mardegan Issa

L R Missana

M Alonso

J C Rodriguez-Cabello

S Feldman

Affiliations

Soon will be listed here.

Abstract

Large bone defects are a significant health problem today with various origins, including extensive trauma, tumours, or congenital musculoskeletal disorders. Tissue engineering, and in particular bone tissue engineering, aims to respond to this demand. As such, we propose a specific model based on Elastin-Like Recombinamers-based click-chemistry hydrogels given their high biocompatibility and their potent on bone regeneration effect conferred by different bioactive sequences. In this work we demonstrate, using biochemistry, histology, histomorphometry and imaging techniques, the biocompatibility of our matrix and its potent effect on bone regeneration in a model of bone parietal lesion in female New Zealand rabbits.

References

Ruoslahti E . RGD and other recognition sequences for integrins. Annu Rev Cell Dev Biol. 1996; 12:697-715. DOI: 10.1146/annurev.cellbio.12.1.697. View

Athanasiou K, Zhu C, Lanctot D, Agrawal C, Wang X . Fundamentals of biomechanics in tissue engineering of bone. Tissue Eng. 2000; 6(4):361-81. DOI: 10.1089/107632700418083. View

Roberts S, Harmon T, Schaal J, Miao V, Li K, Hunt A . Injectable tissue integrating networks from recombinant polypeptides with tunable order. Nat Mater. 2018; 17(12):1154-1163. PMC: 6329288. DOI: 10.1038/s41563-018-0182-6. View

Chung E, Gilbert M, Virdi A, Sena K, Sumner D, Healy K . Biomimetic artificial ECMs stimulate bone regeneration. J Biomed Mater Res A. 2006; 79(4):815-26. DOI: 10.1002/jbm.a.30809. View

Rodriguez-Cabello J, Girotti A, Ribeiro A, Javier Arias F . Synthesis of genetically engineered protein polymers (recombinamers) as an example of advanced self-assembled smart materials. Methods Mol Biol. 2011; 811:17-38. DOI: 10.1007/978-1-61779-388-2_2. View

Saravanan R, Holdbrook D, Petrlova J, Singh S, Berglund N, Choong Y . Structural basis for endotoxin neutralisation and anti-inflammatory activity of thrombin-derived C-terminal peptides. Nat Commun. 2018; 9(1):2762. PMC: 6050251. DOI: 10.1038/s41467-018-05242-0. View

Anada T, Pan C, Stahl A, Mori S, Fukuda J, Suzuki O . Vascularized Bone-Mimetic Hydrogel Constructs by 3D Bioprinting to Promote Osteogenesis and Angiogenesis. Int J Mol Sci. 2019; 20(5). PMC: 6429349. DOI: 10.3390/ijms20051096. View

Jahan K, Tabrizian M . Composite biopolymers for bone regeneration enhancement in bony defects. Biomater Sci. 2015; 4(1):25-39. DOI: 10.1039/c5bm00163c. View

Weibel E, KISTLER G, Scherle W . Practical stereological methods for morphometric cytology. J Cell Biol. 1966; 30(1):23-38. PMC: 2106982. DOI: 10.1083/jcb.30.1.23. View

10.

Xavier M, Farez N, Salvatierra P, Jardini A, Kharmandayan P, Feldman S . Biological performance of a bioabsorbable Poly (L-Lactic Acid) produced in polymerization unit: studies. F1000Res. 2022; 10:1275. PMC: 8729025. DOI: 10.12688/f1000research.73754.1. View

11.

Gonzalez de Torre I, Santos M, Quintanilla L, Testera A, Alonso M, Cabello J . Elastin-like recombinamer catalyst-free click gels: characterization of poroelastic and intrinsic viscoelastic properties. Acta Biomater. 2014; 10(6):2495-505. DOI: 10.1016/j.actbio.2014.02.006. View

12.

Paulini M, Camal Ruggieri I, Ramallo M, Alonso M, Rodriguez-Cabello J, Esbrit P . Recombinant Proteins-Based Strategies in Bone Tissue Engineering. Biomolecules. 2022; 12(1). PMC: 8773742. DOI: 10.3390/biom12010003. View

13.

DeForest C, Sims E, Anseth K . Peptide-Functionalized Click Hydrogels with Independently Tunable Mechanics and Chemical Functionality for 3D Cell Culture. Chem Mater. 2010; 22(16):4783-4790. PMC: 2937999. DOI: 10.1021/cm101391y. View

14.

Contessotto P, Orbanic D, Da Costa M, Jin C, Owens P, Chantepie S . Elastin-like recombinamers-based hydrogel modulates post-ischemic remodeling in a non-transmural myocardial infarction in sheep. Sci Transl Med. 2021; 13(581). DOI: 10.1126/scitranslmed.aaz5380. View

15.

Danhier F, Ansorena E, Silva J, Coco R, Le Breton A, Preat V . PLGA-based nanoparticles: an overview of biomedical applications. J Control Release. 2012; 161(2):505-22. DOI: 10.1016/j.jconrel.2012.01.043. View

16.

Mattisson L, Bojan A, Enocson A . Epidemiology, treatment and mortality of trochanteric and subtrochanteric hip fractures: data from the Swedish fracture register. BMC Musculoskelet Disord. 2018; 19(1):369. PMC: 6186067. DOI: 10.1186/s12891-018-2276-3. View

17.

Ruoslahti E, Pierschbacher M . Arg-Gly-Asp: a versatile cell recognition signal. Cell. 1986; 44(4):517-8. DOI: 10.1016/0092-8674(86)90259-x. View

18.

Pescador D, Ibanez-Fonseca A, Sanchez-Guijo F, Brinon J, Javier Arias F, Muntion S . Regeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogels. J Mater Sci Mater Med. 2017; 28(8):115. DOI: 10.1007/s10856-017-5928-1. View

19.

Costa R, Custodio C, Arias F, Rodriguez-Cabello J, Mano J . Layer-by-layer assembly of chitosan and recombinant biopolymers into biomimetic coatings with multiple stimuli-responsive properties. Small. 2011; 7(18):2640-9. DOI: 10.1002/smll.201100875. View

20.

Klenke F, Liu Y, Yuan H, Hunziker E, Siebenrock K, Hofstetter W . Impact of pore size on the vascularization and osseointegration of ceramic bone substitutes in vivo. J Biomed Mater Res A. 2007; 85(3):777-86. DOI: 10.1002/jbm.a.31559. View