Interaction Between SARS-CoV PBM and Cellular PDZ Domains Leading to Virus Virulence

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2024 Aug 29

PMID 39205188

Authors

Jose M Honrubia

Jose R Valverde

Diego Munoz-Santos

Jorge Ripoll-Gomez

Nuria de la Blanca

Jorge Izquierdo

Marta Villarejo-Torres

Ana Marchena-Pasero

Maria Rueda-Huelamo

Ivan Nombela

Mercedes Ruiz-Yuste

Sonia Zuniga

Isabel Sola

Luis Enjuanes

Affiliations

Soon will be listed here.

Abstract

The interaction between SARS-CoV PDZ-binding motifs (PBMs) and cellular PDZs is responsible for virus virulence. The PBM sequence present in the 3a and envelope (E) proteins of SARS-CoV can potentially bind to over 400 cellular proteins containing PDZ domains. The role of SARS-CoV 3a and E proteins was studied. SARS-CoVs, in which 3a-PBM and E-PMB have been deleted (3a-PBM-/E-PBM-), reduced their titer around one logarithmic unit but still were viable. In addition, the absence of the E-PBM and the replacement of 3a-PBM with that of E did not allow the rescue of SARS-CoV. E protein PBM was necessary for virulence, activating p38-MAPK through the interaction with Syntenin-1 PDZ domain. However, the presence or absence of the homologous motif in the 3a protein, which does not bind to Syntenin-1, did not affect virus pathogenicity. Mutagenesis analysis and in silico modeling were performed to study the extension of the PBM of the SARS-CoV E protein. Alanine and glycine scanning was performed revealing a pair of amino acids necessary for optimum virus replication. The binding of E protein with the PDZ2 domain of the Syntenin-1 homodimer induced conformational changes in both PDZ domains 1 and 2 of the dimer.

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