Overexpression of a '' MYB Factor Gene, , Increases Salt and Drought Tolerance in
Overview
Affiliations
is a Sieb.et tree of the family Scrophulariaceae. It has become an important short-to-medium-term fast-growing multi-purpose tree species in China due to its rapid growth, strong adaptability, and excellent material properties. MYB transcription factors in plants have numerous and diverse functions, playing important roles in various aspects such as plant stress response. To investigate the function of MYB transcription factors in , this study used PCR technology to clone the gene from . The homology of PfMYB44 and SiMYB44 () was the highest. Expression analysis results showed that was expressed in the root, stem, young leaf, and mature leaf of , with the highest content in the root. Cold, drought, hot, salt, and ABA treatments could increase the expression level of . Overexpression- plants were constructed, and physiological and molecular analysis showed that could positively regulate salt and drought stresses. Under drought stress, the expression levels of , , and in transgenic lines were significantly induced. Salt stress induced the expression of , , and genes, and the relative expression levels of these genes in transgenic were higher. In conclusion, the functional study of laid a certain foundation for the study of stress resistance, and was helpful to the study of its stress resistance mechanism and the cultivation of new stress resistance varieties.
Singh A, Pal P, Sahoo U, Sharma L, Pandey B, Prakash A Int J Mol Sci. 2024; 25(23).
PMID: 39684248 PMC: 11640768. DOI: 10.3390/ijms252312537.