Comparative Effectiveness of Various Multi-Antigen Vaccines in Controlling in Broiler Chickens

Overview

Journal Vaccines (Basel)

Date 2024 Aug 29

PMID 39204034

Authors

Mostafa Naguib

Shreeya Sharma

Abigail Schneider

Sarah Wehmueller

Khaled Abdelaziz

Affiliations

Soon will be listed here.

Abstract

This study was undertaken to evaluate and compare the efficacy of different multi-antigen vaccines, including heat-inactivated, whole lysate, and subunit (outer membrane proteins [OMPs]) vaccines along with the immunostimulant CpG ODN in controlling colonization in chickens. In the first trial, 125 μg of OMPs and 50 μg of CpG ODN were administered individually or in combination, either in ovo to chick embryos or subcutaneously (SC) to one-day-old chicks. In the second trial, different concentrations of antigens (heat-killed, whole lysate, and OMPs) were administered SC to one-day-old chicks. The results of the first trial revealed that SC immunization with the combination of CpG ODN and OMPs elevated interferon (IFN)-γ, interleukin (IL)-1β, and IL-13 gene expression in the spleen, significantly increased serum IgM and IgY antibody levels, and reduced cecal counts by approximately 1.2 log. In contrast, in ovo immunization did not elicit immune responses or confer protection against . The results of the second trial showed that SC immunization with whole lysate or 200 μg OMPs reduced counts by approximately 1.4 and 1.1 log, respectively. In conclusion, lysate and OMPs are promising vaccine antigens for reducing colonization in chickens.

Citing Articles

A multi-antigen Campylobacter vaccine enhances antibody responses in layer breeders and sustains elevated maternal antibody levels in their offspring.

Naguib M, Sharma S, Schneider A, Bragg A, Abdelaziz K Poult Sci. 2025; 104(4):104898.

PMID: 39985894 PMC: 11904490. DOI: 10.1016/j.psj.2025.104898.

References

Abdul-Careem M, Hunter D, Lambourne M, Barta J, Sharif S . Ontogeny of cytokine gene expression in the chicken spleen. Poult Sci. 2007; 86(7):1351-5. DOI: 10.1093/ps/86.7.1351. View

McCOY E, Doyle D, Burda K, Corbeil L, Winter A . Superficial antigens of Campylobacter (Vibrio) fetus: characterization of antiphagocytic component. Infect Immun. 1975; 11(3):517-25. PMC: 415096. DOI: 10.1128/iai.11.3.517-525.1975. View

Vandeputte J, Martel A, Van Rysselberghe N, Antonissen G, Verlinden M, De Zutter L . In ovo vaccination of broilers against Campylobacter jejuni using a bacterin and subunit vaccine. Poult Sci. 2019; 98(11):5999-6004. DOI: 10.3382/ps/pez402. View

Taha-Abdelaziz K, Singh M, Sharif S, Sharma S, Kulkarni R, Alizadeh M . Intervention Strategies to Control at Different Stages of the Food Chain. Microorganisms. 2023; 11(1). PMC: 9866650. DOI: 10.3390/microorganisms11010113. View

Gunawardana T, Ahmed K, Goonewardene K, Popowich S, Kurukulasuriya S, Karunarathna R . Synthetic CpG-ODN rapidly enriches immune compartments in neonatal chicks to induce protective immunity against bacterial infections. Sci Rep. 2019; 9(1):341. PMC: 6344490. DOI: 10.1038/s41598-018-36588-6. View

Gunawardana T, Foldvari M, Zachar T, Popowich S, Chow-Lockerbie B, Ivanova M . Protection of Neonatal Broiler Chickens Following in ovo Delivery of Oligodeoxynucleotides Containing CpG Motifs (CpG-ODN) Formulated with Carbon Nanotubes or Liposomes. Avian Dis. 2015; 59(1):31-7. DOI: 10.1637/10832-032814-reg. View

Junttila I . Tuning the Cytokine Responses: An Update on Interleukin (IL)-4 and IL-13 Receptor Complexes. Front Immunol. 2018; 9:888. PMC: 6001902. DOI: 10.3389/fimmu.2018.00888. View

Gimeno I, Faiz N, Cortes A, Barbosa T, Villalobos T, Pandiri A . In Ovo Vaccination with Turkey Herpesvirus Hastens Maturation of Chicken Embryo Immune Responses in Specific-Pathogen-Free Chickens. Avian Dis. 2015; 59(3):375-83. DOI: 10.1637/11060-031115-Reg.1. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

10.

Ng L, Sutherland A, Newton R, Qian F, Cachero T, Scott M . B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells. J Immunol. 2004; 173(2):807-17. DOI: 10.4049/jimmunol.173.2.807. View

11.

Taghavi A, Allan B, Mutwiri G, Van Kessel A, Willson P, Babiuk L . Protection of neonatal broiler chicks against Salmonella Typhimurium septicemia by DNA containing CpG motifs. Avian Dis. 2008; 52(3):398-406. DOI: 10.1637/8196-121907-Reg. View

12.

El-Shibiny A, Connerton P, Connerton I . Enumeration and diversity of campylobacters and bacteriophages isolated during the rearing cycles of free-range and organic chickens. Appl Environ Microbiol. 2005; 71(3):1259-66. PMC: 1065130. DOI: 10.1128/AEM.71.3.1259-1266.2005. View

13.

Taha-Abdelaziz K, Alkie T, Hodgins D, Yitbarek A, Shojadoost B, Sharif S . Gene expression profiling of chicken cecal tonsils and ileum following oral exposure to soluble and PLGA-encapsulated CpG ODN, and lysate of Campylobacter jejuni. Vet Microbiol. 2017; 212:67-74. DOI: 10.1016/j.vetmic.2017.11.010. View

14.

Ricks C, Avakian A, Bryan T, Gildersleeve R, Haddad E, Ilich R . In ovo vaccination technology. Adv Vet Med. 1999; 41:495-515. View

15.

Annamalai T, Pina-Mimbela R, Kumar A, Binjawadagi B, Liu Z, Renukaradhya G . Evaluation of nanoparticle-encapsulated outer membrane proteins for the control of Campylobacter jejuni colonization in chickens. Poult Sci. 2013; 92(8):2201-11. DOI: 10.3382/ps.2012-03004. View

16.

St Paul M, Mallick A, Haq K, Orouji S, Abdul-Careem M, Sharif S . In vivo administration of ligands for chicken toll-like receptors 4 and 21 induces the expression of immune system genes in the spleen. Vet Immunol Immunopathol. 2011; 144(3-4):228-37. DOI: 10.1016/j.vetimm.2011.09.004. View

17.

McGruder E, Ramirez G, Kogut M, Moore R, Corrier D, Deloach J . In ovo administration of Salmonella enteritidis-immune lymphokines confers protection to neonatal chicks against Salmonella enteritidis organ infectivity. Poult Sci. 1995; 74(1):18-25. DOI: 10.3382/ps.0740018. View

18.

Taha-Abdelaziz K, Astill J, Shojadoost B, Borrelli S, Monteiro M, Sharif S . Campylobacter-derived ligands induce cytokine and chemokine expression in chicken macrophages and cecal tonsil mononuclear cells. Vet Microbiol. 2020; 246:108732. DOI: 10.1016/j.vetmic.2020.108732. View

19.

Alizadeh M, Shojadoost B, Astill J, Taha-Abdelaziz K, Karimi S, Bavananthasivam J . Effects of Inoculation of Multi-Strain Lactobacilli on Cytokine Gene Expression and Antibody-Mediated Immune Responses in Chickens. Front Vet Sci. 2020; 7:105. PMC: 7058628. DOI: 10.3389/fvets.2020.00105. View

20.

Helmy Y, Taha-Abdelaziz K, Hawwas H, Ghosh S, Sami Alkafaas S, Moawad M . Antimicrobial Resistance and Recent Alternatives to Antibiotics for the Control of Bacterial Pathogens with an Emphasis on Foodborne Pathogens. Antibiotics (Basel). 2023; 12(2). PMC: 9952301. DOI: 10.3390/antibiotics12020274. View