Response Surface Methodology-Based Optimization of the Chitinolytic Activity of Strain 614 Exerting Biological Control Against Phytopathogenic Fungi

Overview

Journal Microorganisms

Specialty Microbiology

Date 2024 Aug 29

PMID 39203422

Authors

Imen Ben Slimene Debez

Hayet Houmani

Henda Mahmoudi

Khaoula Mkadmini

Pedro Garcia-Caparros

Ahmed Debez

Olfa Tabbene

Naceur Djebali

Maria-Camino Urdaci

Affiliations

Soon will be listed here.

Abstract

As part of the development of alternative and environmentally friendly control against phytopathogenic fungi, could be a useful species notably via the generation of hydrolytic enzymes like chitinases, which can act as a biological control agent. Here, a S614 strain exhibiting chitinase activity was isolated from a soil in southern Tunisia. Then, response surface methodology (RSM) with a central composite design (CCD) was used to assess the impact of five factors (colloidal chitin, magnesium sulfate, dipotassium phosphate, yeast extract, and ammonium sulfate) on chitinase activity. strain 614 growing in the optimized medium showed up to a 3-fold higher chitinase activity. This enzyme was identified as beta-N-acetylhexosaminidase (90.1 kDa) based on its peptide sequences, which showed high similarity to those of strain 383. Furthermore, this chitinase significantly inhibited the growth of two phytopathogenic fungi: M5 and Ph8. Interestingly, a crude enzyme from strain S614 was effective in reducing damage on detached leaves of . Overall, our data provide strong arguments for the agricultural and biotechnological potential of strain S614 in the context of developing biocontrol approaches.

References

Tagele S, Kim S, Lee H, Kim H, Lee Y . Effectiveness of multi-trait Burkholderia contaminans KNU17BI1 in growth promotion and management of banded leaf and sheath blight in maize seedling. Microbiol Res. 2018; 214:8-18. DOI: 10.1016/j.micres.2018.05.004. View

Techkarnjanaruk S, Pongpattanakitshote S, Goodman A . Use of a promoterless lacZ gene insertion to investigate chitinase gene expression in the marine bacterium Pseudoalteromonas sp. strain S9. Appl Environ Microbiol. 1997; 63(8):2989-96. PMC: 168598. DOI: 10.1128/aem.63.8.2989-2996.1997. View

Cardozo F, Feitosa V, Mendonca C, Santos da Silva F, Converti A, Pinheiro de Souza Oliveira R . Enhanced production of N-acetyl-glucosaminidase by marine Aeromonas caviae CHZ306 in bioreactor. Braz J Microbiol. 2023; 54(3):1533-1545. PMC: 10485184. DOI: 10.1007/s42770-023-01088-x. View

Chen F, Chen X, Qin L, Tao Y, Dong Z . Characterization and homologous overexpression of an N-acetylglucosaminidase Nag1 from Trichoderma reesei. Biochem Biophys Res Commun. 2014; 459(2):184-188. DOI: 10.1016/j.bbrc.2014.12.066. View

Ben Slimene I, Tabbene O, Gharbi D, Mnasri B, Schmitter J, Urdaci M . Isolation of a chitinolytic Bacillus licheniformis S213 strain exerting a biological control against Phoma medicaginis infection. Appl Biochem Biotechnol. 2015; 175(7):3494-506. DOI: 10.1007/s12010-015-1520-7. View

Bassler B, Yu C, Lee Y, Roseman S . Chitin utilization by marine bacteria. Degradation and catabolism of chitin oligosaccharides by Vibrio furnissii. J Biol Chem. 1991; 266(36):24276-86. View

Liu T, Duan Y, Yang Q . Revisiting glycoside hydrolase family 20 β-N-acetyl-d-hexosaminidases: Crystal structures, physiological substrates and specific inhibitors. Biotechnol Adv. 2018; 36(4):1127-1138. DOI: 10.1016/j.biotechadv.2018.03.013. View

Qu T, Zhang C, Qin Z, Fan L, Jiang L, Zhao L . A Novel GH Family 20 β-N-acetylhexosaminidase With Both Chitosanase and Chitinase Activity From . Front Mol Biosci. 2021; 8:684086. PMC: 8170477. DOI: 10.3389/fmolb.2021.684086. View

Depoorter E, Bull M, Peeters C, Coenye T, Vandamme P, Mahenthiralingam E . Burkholderia: an update on taxonomy and biotechnological potential as antibiotic producers. Appl Microbiol Biotechnol. 2016; 100(12):5215-29. DOI: 10.1007/s00253-016-7520-x. View

10.

Philip N, Koteshwara A, Kiran G, Raja S, Subrahmanyam V, Chandrashekar H . Statistical Optimization for Coproduction of Chitinase and Beta 1, 4-Endoglucanase by Chitinolytic Paenibacillus elgii PB1 Having Antifungal Activity. Appl Biochem Biotechnol. 2020; 191(1):135-150. DOI: 10.1007/s12010-020-03235-8. View

11.

Wahab W, Esawy M . Statistical, physicochemical, and thermodynamic profiles of chitinase production from local agro-industrial wastes employing the honey isolate EM77. Heliyon. 2022; 8(10):e10869. PMC: 9561733. DOI: 10.1016/j.heliyon.2022.e10869. View

12.

Jin Y, Zhou J, Zhou J, Hu M, Zhang Q, Kong N . Genome-based classification of Burkholderia cepacia complex provides new insight into its taxonomic status. Biol Direct. 2020; 15(1):6. PMC: 7057466. DOI: 10.1186/s13062-020-0258-5. View

13.

Vaghela B, Vashi R, Rajput K, Joshi R . Plant chitinases and their role in plant defense: A comprehensive review. Enzyme Microb Technol. 2022; 159:110055. DOI: 10.1016/j.enzmictec.2022.110055. View

14.

Ajavakom A, Supsvetson S, Somboot A, Sukwattanasinitt M . Products from microwave and ultrasonic wave assisted acid hydrolysis of chitin. Carbohydr Polym. 2014; 90(1):73-7. DOI: 10.1016/j.carbpol.2012.04.064. View

15.

Shahrajabian M, Chaski C, Polyzos N, Tzortzakis N, Petropoulos S . Sustainable Agriculture Systems in Vegetable Production Using Chitin and Chitosan as Plant Biostimulants. Biomolecules. 2021; 11(6). PMC: 8226918. DOI: 10.3390/biom11060819. View

16.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

17.

Li X, Roseman S . The chitinolytic cascade in Vibrios is regulated by chitin oligosaccharides and a two-component chitin catabolic sensor/kinase. Proc Natl Acad Sci U S A. 2003; 101(2):627-31. PMC: 327198. DOI: 10.1073/pnas.0307645100. View

18.

Singh R, Praveen Kumar D, Solanki M, Singh P, Srivastva A, Kumar S . Optimization of media components for chitinase production by chickpea rhizosphere associated Lysinibacillus fusiformis B-CM18. J Basic Microbiol. 2012; 53(5):451-60. DOI: 10.1002/jobm.201100590. View

19.

Cd D, Lb V, Ma M, Md B . Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats. Curr Microbiol. 2021; 78(7):2787-2798. DOI: 10.1007/s00284-021-02555-0. View

20.

Kazami N, Sakaguchi M, Mizutani D, Masuda T, Wakita S, Oyama F . A simple procedure for preparing chitin oligomers through acetone precipitation after hydrolysis in concentrated hydrochloric acid. Carbohydr Polym. 2015; 132:304-10. DOI: 10.1016/j.carbpol.2015.05.082. View