Anti-TCP1 Antibody Is a Potential Biomarker for Diagnosing Systemic Lupus Erythematosus

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Aug 29

PMID 39201300

Authors

Sang-Won Lee

Wook-Young Baek

So-Won Park

Jee-Min Chung

Ji-Hyun Park

Ho Chul Kang

Ju-Yang Jung

Chang-Hee Suh

Affiliations

Soon will be listed here.

Abstract

Systemic lupus erythematosus (SLE) is a chronic inflammatory disease caused by autoantibodies. Serum samples from patients with SLE ( = 10) were compared with those from normal controls (NCs, = 5) using 21K protein chip analysis to identify a biomarker for SLE, revealing 63 SLE-specific autoantibodies. The anti-chaperonin-containing t-complex polypeptide-1 (TCP1) antibody exhibited higher expression in patients with SLE than in NCs. To validate the specificity of the anti-TCP1 antibody in SLE, dot blot analysis was conducted using sera from patients with SLE ( = 100), rheumatoid arthritis (RA; = 25), Behçet's disease (BD; = 28), and systemic sclerosis (SSc; = 30) and NCs ( = 50). The results confirmed the detection of anti-TCP1 antibodies in 79 of 100 patients with SLE, with substantially elevated expression compared to both NCs and patients with other autoimmune diseases. We performed an enzyme-linked immunosorbent assay to determine the relative amounts of anti-TCP1 antibodies; markedly elevated anti-TCP1 antibody levels were detected in the sera of patients with SLE (50.1 ± 17.3 arbitrary unit (AU), = 251) compared to those in NCs (33.9 ± 9.3 AU), RA (35 ± 8.7 AU), BD (37.5 ± 11.6 AU), and SSc (43 ± 11.9 AU). These data suggest that the anti-TCP1 antibody is a potential diagnostic biomarker for SLE.

References

Fava A, Petri M . Systemic lupus erythematosus: Diagnosis and clinical management. J Autoimmun. 2018; 96:1-13. PMC: 6310637. DOI: 10.1016/j.jaut.2018.11.001. View

Hu C, Huang W, Chen H, Song G, Li P, Shan Q . Autoantibody profiling on human proteome microarray for biomarker discovery in cerebrospinal fluid and sera of neuropsychiatric lupus. PLoS One. 2015; 10(5):e0126643. PMC: 4425696. DOI: 10.1371/journal.pone.0126643. View

Petri M, Orbai A, Alarcon G, Gordon C, Merrill J, Fortin P . Derivation and validation of the Systemic Lupus International Collaborating Clinics classification criteria for systemic lupus erythematosus. Arthritis Rheum. 2012; 64(8):2677-86. PMC: 3409311. DOI: 10.1002/art.34473. View

Zeinab M, Hiam K, Edmond C, Assem E . Selective adsorption of antiphospholipid and anti-dsDNA autoantibodies on histidine based pseudobioaffinity adsorbent from sera of patients with systemic lupus erythematosus (SLE). J Chromatogr B Analyt Technol Biomed Life Sci. 2014; 975:77-83. DOI: 10.1016/j.jchromb.2014.11.005. View

Ahmad A, Imran M, Ahsan H . Biomarkers as Biomedical Bioindicators: Approaches and Techniques for the Detection, Analysis, and Validation of Novel Biomarkers of Diseases. Pharmaceutics. 2023; 15(6). PMC: 10303887. DOI: 10.3390/pharmaceutics15061630. View

Aringer M, Costenbader K, Daikh D, Brinks R, Mosca M, Ramsey-Goldman R . 2019 European League Against Rheumatism/American College of Rheumatology Classification Criteria for Systemic Lupus Erythematosus. Arthritis Rheumatol. 2019; 71(9):1400-1412. PMC: 6827566. DOI: 10.1002/art.40930. View

Sutandy F, Qian J, Chen C, Zhu H . Overview of protein microarrays. Curr Protoc Protein Sci. 2013; Chapter 27:Unit 27.1. PMC: 3680110. DOI: 10.1002/0471140864.ps2701s72. View

Ahmadpoor P, Dalili N, Rostami M . An update on pathogenesis of systemic lupus erythematosus. Iran J Kidney Dis. 2014; 8(3):171-84. View

Naganuma T, Shiogama K, Uchiumi T . The N-terminal regions of eukaryotic acidic phosphoproteins P1 and P2 are crucial for heterodimerization and assembly into the ribosomal GTPase-associated center. Genes Cells. 2007; 12(4):501-10. DOI: 10.1111/j.1365-2443.2007.01067.x. View

10.

Aletaha D, Neogi T, Silman A, Funovits J, Felson D, Bingham 3rd C . 2010 rheumatoid arthritis classification criteria: an American College of Rheumatology/European League Against Rheumatism collaborative initiative. Ann Rheum Dis. 2010; 69(9):1580-8. DOI: 10.1136/ard.2010.138461. View

11.

Poetz O, Schwenk J, Kramer S, Stoll D, Templin M, Joos T . Protein microarrays: catching the proteome. Mech Ageing Dev. 2004; 126(1):161-70. DOI: 10.1016/j.mad.2004.09.030. View

12.

Su D, Liu R, Li X, Sun L . Possible novel biomarkers of organ involvement in systemic lupus erythematosus. Clin Rheumatol. 2014; 33(8):1025-31. DOI: 10.1007/s10067-014-2560-z. View

13.

Pisetsky D . Pathogenesis of autoimmune disease. Nat Rev Nephrol. 2023; 19(8):509-524. PMC: 10171171. DOI: 10.1038/s41581-023-00720-1. View

14.

Huang Y, Zhu H . Protein Array-based Approaches for Biomarker Discovery in Cancer. Genomics Proteomics Bioinformatics. 2017; 15(2):73-81. PMC: 5414965. DOI: 10.1016/j.gpb.2017.03.001. View

15.

Han W, Jin M, Liu C, Zhao Q, Wang S, Wang Y . Structural basis of plp2-mediated cytoskeletal protein folding by TRiC/CCT. Sci Adv. 2023; 9(11):eade1207. PMC: 10017041. DOI: 10.1126/sciadv.ade1207. View

16.

Ma H, Murphy C, Loscher C, OKennedy R . Autoantibodies - enemies, and/or potential allies?. Front Immunol. 2022; 13:953726. PMC: 9627499. DOI: 10.3389/fimmu.2022.953726. View

17.

Artero-Castro A, Perez-Alea M, Feliciano A, Leal J, Genestar M, Castellvi J . Disruption of the ribosomal P complex leads to stress-induced autophagy. Autophagy. 2015; 11(9):1499-519. PMC: 4590587. DOI: 10.1080/15548627.2015.1063764. View

18.

Esdaile J, Abrahamowicz M, Mackenzie T, HAYSLETT J, Kashgarian M . The time-dependence of long-term prediction in lupus nephritis. Arthritis Rheum. 1994; 37(3):359-68. DOI: 10.1002/art.1780370309. View

19.

Chung J, Jung Y, Kim Y, Song J, Kim S, Kim J . Identification of the Thioredoxin-Like 2 Autoantibody as a Specific Biomarker for Triple-Negative Breast Cancer. J Breast Cancer. 2018; 21(1):87-90. PMC: 5880970. DOI: 10.4048/jbc.2018.21.1.87. View

20.

Kubota H, Hynes G, Willison K . The chaperonin containing t-complex polypeptide 1 (TCP-1). Multisubunit machinery assisting in protein folding and assembly in the eukaryotic cytosol. Eur J Biochem. 1995; 230(1):3-16. DOI: 10.1111/j.1432-1033.1995.tb20527.x. View