Electron Microscopical Analysis of Drosophila Polytene Chromosomes. II. Development of Complex Puffs

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 1985 Jan 1

PMID 3920013

Citations 10

Authors

V F Semeshin

e m Baricheva

E S Belyaeva

I F Zhimulev

Affiliations

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Abstract

Data are presented of electron microscopic (EM) analysis of consecutive developmental stages of Drosophila melanogaster complex puffs, formed as a result of simultaneous decondensation of several bands. EM mapping principles proposed by us permitted more exact determination of the banding patterns of 19 regions in which 31 puffs develop. It is shown that 20 of them develop as a result of synchronous decondensation of two bands, 7 of three and 4 of one band. Three cases of two-band puff formation when one or both bands undergo partial decondensation are described. In the 50CF, 62CE, 63F and 71CF regions puffing zones are located closely adjacent to each other but the decondensation of separate band groups occurs at different puff stages (PS). These data are interpreted as activation of independently regulated DNA sequences. The decondensation of two or three adjacent bands during formation of the majority of the puffs occurs simultaneously in the very first stages of their development. It demonstrates synchronous activation of the material of several bands presumably affected by a common inductor. Bands adjacent to puffing centres also lose their clarity as the puff develops, probably due to "passive" decondensation connected with puff growth. The morphological data obtained suggest a complex genetic organisation of many puffs.

Citing Articles

Genes Containing Long Introns Occupy Series of Bands and Interbands In polytene Chromosomes.

Khoroshko V, Pokholkova G, Levitsky V, Zykova T, Antonenko O, Belyaeva E Genes (Basel). 2020; 11(4).

PMID: 32290448 PMC: 7230524. DOI: 10.3390/genes11040417.

Three-Dimensional Genome Organization and Function in Drosophila.

Schwartz Y, Cavalli G Genetics. 2017; 205(1):5-24.

PMID: 28049701 PMC: 5223523. DOI: 10.1534/genetics.115.185132.

Localization and characteristics of DNA underreplication zone in the 75C region of intercalary heterochromatin in Drosophila melanogaster polytene chromosomes.

Andreyenkova N, Kokoza E, Semeshin V, Belyaeva E, Demakov S, Pindyurin A Chromosoma. 2009; 118(6):747-61.

PMID: 19685068 DOI: 10.1007/s00412-009-0232-6.

Electron microscopic in situ hybridization of digoxigenin-dUTP-labelled DNA probes with Drosophila melanogaster polytene chromosomes.

Semeshin V, Artero R, Perez-Alonso M, Shloma V Chromosome Res. 1999; 6(5):405-10.

PMID: 9872670 DOI: 10.1023/a:1009233524443.

Electron microscopical analysis of Drosophila polytene chromosomes. III. Mapping of puffs developing from one band.

Semeshin V, Baricheva E, Belyaeva E, Zhimulev I Chromosoma. 1985; 91(3-4):234-50.

PMID: 3920014 DOI: 10.1007/BF00328219.

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