Centrosomal Protein 55 Regulates Chromosomal Instability in Cancer Cells by Controlling Microtubule Dynamics

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2024 Aug 28

PMID 39195269

Authors

Stefanie Muhs

Themistoklis Paraschiakos

Paula Schafer

Simon A Joosse

Sabine Windhorst

Affiliations

Soon will be listed here.

Abstract

Centrosomal Protein 55 (CEP55) exhibits various oncogenic activities; it regulates the PI3K-Akt-pathway, midbody abscission, and chromosomal instability (CIN) in cancer cells. Here, we analyzed the mechanism of how CEP55 controls CIN in ovarian and breast cancer (OvCa) cells. Down-regulation of CEP55 reduced CIN in all cell lines analyzed, and CEP55 depletion decreased spindle microtubule (MT)-stability in OvCa cells. Moreover, recombinant CEP55 accelerated MT-polymerization and attenuated cold-induced MT-depolymerization. To analyze a potential relationship between CEP55-controlled CIN and its impact on MT-stability, we identified the CEP55 MT-binding peptides inside the CEP55 protein. Thereafter, a mutant with deficient MT-binding activity was re-expressed in CEP55-depleted OvCa cells and we could show that this mutant did not restore reduced CIN in CEP55-depleted cells. This finding strongly indicates that CEP55 regulates CIN by controlling MT dynamics.

Citing Articles

Mechanism of Centrosomal Protein 55 (CEP55) Loading Into Exosomes.

Dahlstroem C, Barezani J, Li J, Sopelniak K, Muhs S, Schneider C J Extracell Vesicles. 2025; 14(2):e70046.

PMID: 39976236 PMC: 11840697. DOI: 10.1002/jev2.70046.

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