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Evaluation of Th1/Th2/Th17 Balance in Pulmonary Cystic Echinococcosis Patients

Overview
Journal Acta Parasitol
Specialty Parasitology
Date 2024 Aug 27
PMID 39190279
Authors
Umut Gazi
Yunus Emre Beyhan
Ozgur Tosun
Djursun Karasartova
Ufuk Cobanoglu
Aysegul Taylan-Ozkan
Affiliations
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Abstract

Purpose: Cystic echinococcosis (CE) is a neglected tropical disease prevalent worldwide, particularly in rural areas. Previous studies evaluated immune responses in patients with hepatic CE, however none had assessed Th1, Th2 and Th17 levels simultaneously in pulmonary CE patients. This study aimed to fill this gap in literature by using flow cytometry analysis.

Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples collected from healthy control (HC) volunteers and patients with active pulmonary CE cysts. The PBMCs were analysed to evaluate Th1, Th2, and Th17 cell levels within the CD3 + CD4 + T-cell population, using antibodies against interferon (IFN)-γ, interleukin (IL)-4, and IL-17, respectively.

Results: Our analysis revealed elevated Th2 levels in CE patients, while Th1 and Th17 cell counts showed no significant difference between HC volunteers and patients with pulmonary CE.

Conclusion: The results indicate an imbalanced Th1/Th2/Th17 cell regulation in the pathogenesis of pulmonary CE. Future studies are recommended to compare immune responses between pulmonary and hepatic CE to confirm these findings and evaluate any potential difference in the immunopathology associated with the two clinical forms of CE.

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