Alginate Oligosaccharides Exert Protective Effects on Hydrogen Peroxide-induced Senescence in H9C2 Cardiomyocytes by Regulating the Redox State of Cells

Overview

Journal Food Sci Biotechnol

Specialty Biotechnology

Date 2024 Aug 26

PMID 39184993

Authors

Wen-Jing Feng

Jie Mou

Ping-Ping Liao

Jing Zhou

Nan-Nan Zhang

Ting Hu

Shan Wang

Shi-Yuan Zhang

Yong-Jun Mao

Affiliations

Soon will be listed here.

Abstract

Aging is a known independent risk factor for several cardiovascular diseases. Here, we evaluated potential effects and possible mechanisms through which alginate oligosaccharides (AOS) affect hydrogen peroxide (HO)-induced senescence in H9C2 cardiomyocytes. A series of AOS molecules, including oligoM, oligoG, M-5, and G-5, were investigated. AOS significantly decreased SA-β-gal and DAPI-stained positive cells, downregulated p53 and p21 (aging-related markers) expression, and eventually protected H9C2 cells from HO-induced senescence. AOS decreased reactive oxygen species and malondialdehyde production, recovered mitochondrial function, and alleviated the oxidative stress state by regulating PGC-1α and NADPH oxidase subunit expression. Furthermore, AOS treatment restored the expression of antioxidant enzymes in senescent H9C2 cells. Thus, our results show in vitro evidence that AOS alleviate senescence in H9C2 cells by regulating the redox state; thus, AOS may be an effective therapeutic agent that could protect against cardiomyocyte senescence.

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