Low Expression of Lnc-ENST00000535078 Inhibits the Migration, Invasion, and Enhances Apoptosis of CTPE-induced Malignantly Transformed BEAS-2B Cells

Overview

Journal Toxicol Res (Camb)

Publisher Oxford University Press

Date 2024 Aug 23

PMID 39175813

Authors

Ping Lu

Liu Yang

Yanting Lei

Yuezeng Zhao

Zhihao Tang

Pingping Shang

Xiaolei Zhou

Pengpeng Wang

Wei Wang

Feifei Feng

Qiao Zhang

Affiliations

Soon will be listed here.

Abstract

Long non-coding RNA (LncRNA) plays an important role in malignant transformation of cells. This study aimed to explore the role of Lnc-ENST00000535078 in the malignant transformation of immortalized human bronchial epithelial cells (BEAS-2B) induced by coal tar pitch extract (CTPE). The malignant transformation model of BEAS-2B cells exposed to CTPE. Cell proliferation was examined by Cell counting kit-8 (CCK-8) assay. Colony formation assay was used to assess the colony of cells. Cell migration and invasion were detected by Transwell analysis. Cell cycle progression and apoptotic status were assessed by flow cytometry. Differentially expressed genes were screened by RNA sequencing. The results showed that Lnc-ENST00000535078 expression was highest in malignantly transformed BEAS-2B cells passaged to the 30th generation. Knockdown of Lnc-ENST00000535078 inhibited the migration, invasion and anti-apoptotic abilities of malignantly transformed BEAS-2B cells. Transcriptome analysis found 608 differential genes. CCND1 and FOS genes were screened out because of their levels were positive correlation with the expression of Lnc-ENST00000535078, which were consistent with the RNA sequencing results. In conclusion, Low expression of Lnc-ENST00000535078 inhibits the migration and invasion of malignant transformed BEAS-2B cells and promotes apoptosis in these cells. Lnc-ENST00000556926 might affect the malignant transformation of cells through the regulation of CCND1 and FOS. This study may provide a potential target for intervention in CTPE-induced lung cancer.

References

Ding Y, Hao K, Li Z, Ma R, Zhou Y, Zhou Z . c-Fos separation from Lamin A/C by GDF15 promotes colon cancer invasion and metastasis in inflammatory microenvironment. J Cell Physiol. 2019; 235(5):4407-4421. DOI: 10.1002/jcp.29317. View

Ghafouri-Fard S, Hussen B, Gharebaghi A, Eghtedarian R, Taheri M . LncRNA signature in colorectal cancer. Pathol Res Pract. 2021; 222:153432. DOI: 10.1016/j.prp.2021.153432. View

Zhou L, Zhu Y, Sun D, Zhang Q . Emerging Roles of Long non-coding RNAs in The Tumor Microenvironment. Int J Biol Sci. 2020; 16(12):2094-2103. PMC: 7294937. DOI: 10.7150/ijbs.44420. View

Deng Y, Zhang L . LncRNA SNHG11 accelerates the progression of lung adenocarcinoma via activating Notch pathways. Pathol Res Pract. 2022; 234:153849. DOI: 10.1016/j.prp.2022.153849. View

Ji L, Yang T, Liu M, Li J, Si Q, Wang Y . Construction of lncRNA TYMSOS/hsa-miR-101-3p/CEP55 and TYMSOS/hsa-miR-195-5p/CHEK1 Axis in Non-small Cell Lung Cancer. Biochem Genet. 2022; 61(3):995-1014. DOI: 10.1007/s10528-022-10299-0. View

Yang C, Huang H, Li Y, Zhuo T, Zhu L, Luo C . LncRNA PCAT6 promotes proliferation, migration, invasion, and epithelial-mesenchymal transition of lung adenocarcinoma cell by targeting miR-545-3p. Mol Biol Rep. 2023; 50(4):3557-3568. PMC: 10042954. DOI: 10.1007/s11033-023-08259-x. View

Gao L, Mai A, Li X, Lai Y, Zheng J, Yang Q . LncRNA-DQ786227-mediated cell malignant transformation induced by benzo(a)pyrene. Toxicol Lett. 2013; 223(2):205-10. DOI: 10.1016/j.toxlet.2013.09.015. View

Kim E, Rahmawati L, Aziz N, Kim H, Kim J, Kim K . Protection of c-Fos from autophagic degradation by PRMT1-mediated methylation fosters gastric tumorigenesis. Int J Biol Sci. 2023; 19(12):3640-3660. PMC: 10411464. DOI: 10.7150/ijbs.85126. View

Ma C, Wang D, Tian Z, Gao W, Zang Y, Qian L . USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation. Oncogene. 2023; 42(29):2249-2262. PMC: 10348911. DOI: 10.1038/s41388-023-02739-x. View

10.

Duan S, Wang N, Huang L, Shao H, Zhang P, Wang W . NLRP3 inflammasome activation involved in LPS and coal tar pitch extract-induced malignant transformation of human bronchial epithelial cells. Environ Toxicol. 2019; 34(5):585-593. DOI: 10.1002/tox.22725. View

11.

Zhou M, Lu B, Tan W, Fu M . Identification of lncRNA-miRNA-mRNA regulatory network associated with primary open angle glaucoma. BMC Ophthalmol. 2020; 20(1):104. PMC: 7076920. DOI: 10.1186/s12886-020-01365-5. View

12.

Mu L, Li X, Liu X, Bai H, Peng L, Li Y . Characterization and emission factors of carbonaceous aerosols originating from coke production in China. Environ Pollut. 2020; 268(Pt B):115768. DOI: 10.1016/j.envpol.2020.115768. View

13.

Kim K, Jahan S, Kabir E, Brown R . A review of airborne polycyclic aromatic hydrocarbons (PAHs) and their human health effects. Environ Int. 2013; 60:71-80. DOI: 10.1016/j.envint.2013.07.019. View

14.

Spinelli J, Demers P, Le N, Friesen M, Lorenzi M, Fang R . Cancer risk in aluminum reduction plant workers (Canada). Cancer Causes Control. 2006; 17(7):939-48. DOI: 10.1007/s10552-006-0031-9. View

15.

Osada N, Kikuchi J, Iha H, Yasui H, Ikeda S, Takahashi N . c-FOS is an integral component of the IKZF1 transactivator complex and mediates lenalidomide resistance in multiple myeloma. Clin Transl Med. 2023; 13(8):e1364. PMC: 10426395. DOI: 10.1002/ctm2.1364. View

16.

Xie Z, Zhong C, Shen J, Jia Y, Duan S . LINC00963: A potential cancer diagnostic and therapeutic target. Biomed Pharmacother. 2022; 150:113019. DOI: 10.1016/j.biopha.2022.113019. View

17.

Yuan Y, Yu P, Shen H, Xing G, Li W . LncRNA FOXD2-AS1 Increased Proliferation and Invasion of Lung Adenocarcinoma via Cell-Cycle Regulation. Pharmgenomics Pers Med. 2023; 16:99-109. PMC: 9904230. DOI: 10.2147/PGPM.S396866. View

18.

Friesen M, Demers P, Spinelli J, Eisen E, Lorenzi M, Le N . Chronic and acute effects of coal tar pitch exposure and cardiopulmonary mortality among aluminum smelter workers. Am J Epidemiol. 2010; 172(7):790-9. PMC: 2984260. DOI: 10.1093/aje/kwq208. View

19.

Wang L, Han S, Jin G, Zhou X, Li M, Ying X . Linc00963: a novel, long non-coding RNA involved in the transition of prostate cancer from androgen-dependence to androgen-independence. Int J Oncol. 2014; 44(6):2041-9. DOI: 10.3892/ijo.2014.2363. View

20.

Bridges M, Daulagala A, Kourtidis A . LNCcation: lncRNA localization and function. J Cell Biol. 2021; 220(2). PMC: 7816648. DOI: 10.1083/jcb.202009045. View