A New Raspberry Ketone Synthesis Gene RinPKS4 Identified in Rubus Idaeus L. by Transcriptome Analysis

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2024 Aug 22

PMID 39172993

Authors

Zhimin He

Xihuan Yan

Junxin Zhang

Kang Hu

Mengzhe Ou

Chaojun Wei

Aizhen Yang

Jing Li

Tiran Huang

Mingfeng Yang

Lanqing Ma

Affiliations

Soon will be listed here.

Abstract

Raspberry ketone accounts for the characteristic aroma of the raspberry fruit. In order to explore the genes involved in raspberry ketone synthesis, the transcriptome in fruit tissues of two red raspberry varieties "Polka" and "Orange legend", were sequenced and 24213 single genes were obtained. As the red raspberry fruit ripening, genes involved in flavonoid and anthocyanin synthesis were up-regulated, while those associated with lignin synthesis were down-regulated. A gene (RinPKS4) highly related to raspberry ketone synthesis was identified by transcriptome analysis, and RinPKS4 gene was over-expressed in raspberry in order to further understand the function of RinPKS4 gene in raspberry ketone synthesis. The results showed that the gene expression level of RinPKS4 in the leaf tissues of a transgenic lines increased by about 4-fold and the content of raspberry ketone increased by 42.64% compared with the wide type. This study lays a theoretical foundation for further study on the synthesis and regulation of raspberry ketone in red raspberry.

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