Molecular Detection Using Hybridization Capture and Next-generation Sequencing Reveals Cross-species Transmission of Feline Coronavirus Type-1 Between a Domestic Cat and a Captive Wild Felid

Overview

Journal Microbiol Spectr

Specialty Microbiology

Date 2024 Aug 19

PMID 39158411

Authors

Ximena A Olarte-Castillo

Laura B Goodman

Gary R Whittaker

Affiliations

Soon will be listed here.

Abstract

Importance: Feline coronavirus (FCoV) is highly prevalent in domestic cats worldwide and has also been reported in wild felids, including endangered species, in which it has caused substantial population declines. Characterizing the genetic diversity of FCoV is crucial due to recent reports of novel pathogenic recombinant variants causing high mortality in feral cats in Cyprus. In this retrospective molecular epidemiology study, we used archived samples collected in a zoological institution in the U.S. in which a domestic and a wild felid succumbed to FCoV. Using hybridization capture (HC) and next-generation sequencing, we show for the first time that FCoV can be naturally transmitted between domestic and wild felids. We demonstrate the efficacy of HC for detecting and sequencing the whole genome of FCoV, which is essential to characterize its different genotypes.

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References

Martin D, Rybicki E . RDP: detection of recombination amongst aligned sequences. Bioinformatics. 2000; 16(6):562-3. DOI: 10.1093/bioinformatics/16.6.562. View

Duarte A, Fernandes M, Santos N, Tavares L . Virological Survey in free-ranging wildcats (Felis silvestris) and feral domestic cats in Portugal. Vet Microbiol. 2012; 158(3-4):400-4. PMC: 7117533. DOI: 10.1016/j.vetmic.2012.02.033. View

Xu Y, Kang L, Shen Z, Li X, Wu W, Ma W . Dynamics of severe acute respiratory syndrome coronavirus 2 genome variants in the feces during convalescence. J Genet Genomics. 2021; 47(10):610-617. PMC: 7649052. DOI: 10.1016/j.jgg.2020.10.002. View

Stephenson N, Swift P, Moeller R, Worth S, Foley J . Feline infectious peritonitis in a mountain lion (Puma concolor), California, USA. J Wildl Dis. 2013; 49(2):408-12. DOI: 10.7589/2012-08-210. View

Andre N, Miller A, Whittaker G . Feline infectious peritonitis virus-associated rhinitis in a cat. JFMS Open Rep. 2020; 6(1):2055116920930582. PMC: 7313338. DOI: 10.1177/2055116920930582. View

Tresnan D, Holmes K . Feline aminopeptidase N is a receptor for all group I coronaviruses. Adv Exp Med Biol. 1998; 440:69-75. DOI: 10.1007/978-1-4615-5331-1_9. View

Ketz-Riley C, Ritchey J, Hoover J, Johnson C, Barrie M . Immunodeficiency associated with multiple concurrent infections in captive Pallas' cats (Otocolobus manul). J Zoo Wildl Med. 2003; 34(3):239-45. DOI: 10.1638/01-112. View

Burrows R, Hofer H, East M . Demography, extinction and intervention in a small population: the case of the Serengeti wild dogs. Proc Biol Sci. 1994; 256(1347):281-92. DOI: 10.1098/rspb.1994.0082. View

Martin D, Varsani A, Roumagnac P, Botha G, Maslamoney S, Schwab T . RDP5: a computer program for analyzing recombination in, and removing signals of recombination from, nucleotide sequence datasets. Virus Evol. 2021; 7(1):veaa087. PMC: 8062008. DOI: 10.1093/ve/veaa087. View

10.

Canning G, Camphor H, Schroder B . Rabies outbreak in African Wild Dogs () in the Tuli region, Botswana: Interventions and management mitigation recommendations. J Nat Conserv. 2020; 48:71-76. PMC: 7105255. DOI: 10.1016/j.jnc.2019.02.001. View

11.

Olarte-Castillo X, Dos Remedios J, Heeger F, Hofer H, Karl S, Greenwood A . The virus-host interface: Molecular interactions of Alphacoronavirus-1 variants from wild and domestic hosts with mammalian aminopeptidase N. Mol Ecol. 2021; 30(11):2607-2625. PMC: 8251223. DOI: 10.1111/mec.15910. View

12.

Li H, Durbin R . Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics. 2009; 25(14):1754-60. PMC: 2705234. DOI: 10.1093/bioinformatics/btp324. View

13.

Kokkinaki K, Saridomichelakis M, Mylonakis M, Leontides L, Xenoulis P . Seroprevalence of and risk factors for feline coronavirus infection in cats from Greece. Comp Immunol Microbiol Infect Dis. 2023; 94:101962. DOI: 10.1016/j.cimid.2023.101962. View

14.

Herrewegh A, Smeenk I, Horzinek M, Rottier P, de Groot R . Feline coronavirus type II strains 79-1683 and 79-1146 originate from a double recombination between feline coronavirus type I and canine coronavirus. J Virol. 1998; 72(5):4508-14. PMC: 109693. DOI: 10.1128/JVI.72.5.4508-4514.1998. View

15.

Hohdatsu T, Izumiya Y, Yokoyama Y, Kida K, Koyama H . Differences in virus receptor for type I and type II feline infectious peritonitis virus. Arch Virol. 1998; 143(5):839-50. PMC: 7087195. DOI: 10.1007/s007050050336. View

16.

Addie D, Bellini F, Covell-Ritchie J, Crowe B, Curran S, Fosbery M . Stopping Feline Coronavirus Shedding Prevented Feline Infectious Peritonitis. Viruses. 2023; 15(4). PMC: 10146023. DOI: 10.3390/v15040818. View

17.

Le Poder S . Feline and canine coronaviruses: common genetic and pathobiological features. Adv Virol. 2012; 2011:609465. PMC: 3265309. DOI: 10.1155/2011/609465. View

18.

Tresnan D, Levis R, Holmes K . Feline aminopeptidase N serves as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I. J Virol. 1996; 70(12):8669-74. PMC: 190961. DOI: 10.1128/JVI.70.12.8669-8674.1996. View

19.

Pedersen N . An update on feline infectious peritonitis: diagnostics and therapeutics. Vet J. 2014; 201(2):133-41. PMC: 7110619. DOI: 10.1016/j.tvjl.2014.04.016. View

20.

Li H, Durbin R . Fast and accurate long-read alignment with Burrows-Wheeler transform. Bioinformatics. 2010; 26(5):589-95. PMC: 2828108. DOI: 10.1093/bioinformatics/btp698. View