Oncogenic Fusion Protein Interacts with Polypyrimidine Tract Binding Protein 1 to Facilitate Bladder Cancer Proliferation and Metastasis by Regulating MRNA Stability

Chimeric RNAs, distinct from DNA gene fusions, have emerged as promising therapeutic targets with diverse functions in cancer treatment. However, the functional significance and therapeutic potential of most chimeric RNAs remain unclear. Here we identify a novel fusion transcript of solute carrier family 2-member 11 () and macrophage migration inhibitory factor (). In this study, we investigated the upregulation of in The Cancer Genome Atlas cohort and a cohort of patients from Sun Yat-Sen Memorial Hospital. Subsequently, functional investigations demonstrated that enhanced the proliferation, antiapoptotic effects, and metastasis of bladder cancer cells in vitro and in vivo. Mechanistically, the fusion protein encoded by interacted with polypyrimidine tract binding protein 1 () and regulated the mRNA half-lives of Polo Like Kinase 1, Roundabout guidance receptor 1, and phosphoinositide-3-kinase regulatory subunit 3 in BCa cells. Moreover, knockdown abolished the enhanced impact of on biological function and mRNA stability. Furthermore, the expression of mRNA is regulated by CCCTC-binding factor and stabilized through RNA N4-acetylcytidine modification facilitated by N-acetyltransferase 10. Overall, our findings revealed a significant fusion protein orchestrated by the axis that governs mRNA stability during the multistep progression of bladder cancer.