Training with Reduced Carbohydrate Availability Affects Markers of Bone Resorption and Formation in Male Academy Soccer Players from the English Premier League

Overview

Journal Eur J Appl Physiol

Specialty Physiology

Date 2024 Aug 18

PMID 39154306

Authors

Reuben Stables

Liam Anderson

Craig Sale

Marcus P Hannon

Rachel Dunn

Jonathan C Y Tang

William D Fraser

Nessan B Costello

Graeme L Close

James P Morton

Affiliations

Soon will be listed here.

Abstract

Purpose: To test the hypothesis that training with reduced carbohydrate (CHO) availability increases bone resorption in adolescent soccer players.

Methods: In a randomised crossover design, ten male players (age: 17.4 ± 0.8 years) from an English Premier League academy completed an acute 90-min field-based training session (occurring between 10:30-12:00) in conditions of high (TRAIN HIGH; 1.5 g.kg, 60 g, 1.5 g.kg and 1.5 g.kg consumed at 08:00, during training, 12:30 and 13:30, respectively) or low CHO availability (TRAIN LOW; 0 g.kg). Participants also completed a non-exercise trial (REST) under identical dietary conditions to TRAIN LOW. Venous blood samples were obtained at 08:30, 10:30, 12:30 and 14:30 for assessment of bone resorption (βCTX), bone formation (PINP) and calcium metabolism (PTH and ACa).

Results: External training load did not differ (all P > 0.05) between TRAIN HIGH and TRAIN LOW, as evident for total distance (5.6 ± 0.8; 5.5 ± 0.1 km), average speed (81 ± 9; 85 ± 12 m.min) and high-speed running (350 ± 239; 270 ± 89 m). Area under the curve for both βCTX and PINP was significantly greater (P < 0.01 and P = 0.03) in TRAIN LOW versus TRAIN HIGH, whilst no differences in PTH or ACa (P = 0.11 and P = 0.89) were observed between all three trials.

Conclusion: CHO restriction before, during and after an acute soccer training session increased bone (re)modelling markers in academy players. Despite acute anabolic effects of bone formation, the long-term consequence of bone resorption may impair skeletal development and increase injury risk during growth and maturation.

References

Anderson L, Orme P, Di Michele R, Close G, Morgans R, Drust B . Quantification of training load during one-, two- and three-game week schedules in professional soccer players from the English Premier League: implications for carbohydrate periodisation. J Sports Sci. 2015; 34(13):1250-9. DOI: 10.1080/02640414.2015.1106574. View

Anderson L, Orme P, Di Michele R, Close G, Milsom J, Morgans R . Quantification of Seasonal-Long Physical Load in Soccer Players With Different Starting Status From the English Premier League: Implications for Maintaining Squad Physical Fitness. Int J Sports Physiol Perform. 2016; 11(8):1038-1046. DOI: 10.1123/ijspp.2015-0672. View

Anderson L, Orme P, Naughton R, Close G, Milsom J, Rydings D . Energy Intake and Expenditure of Professional Soccer Players of the English Premier League: Evidence of Carbohydrate Periodization. Int J Sport Nutr Exerc Metab. 2017; 27(3):228-238. DOI: 10.1123/ijsnem.2016-0259. View

Bartlett J, Hawley J, Morton J . Carbohydrate availability and exercise training adaptation: too much of a good thing?. Eur J Sport Sci. 2014; 15(1):3-12. DOI: 10.1080/17461391.2014.920926. View

Bergmann N, Lund A, Gasbjerg L, Jorgensen N, Jessen L, Hartmann B . Separate and Combined Effects of GIP and GLP-1 Infusions on Bone Metabolism in Overweight Men Without Diabetes. J Clin Endocrinol Metab. 2019; 104(7):2953-2960. DOI: 10.1210/jc.2019-00008. View

Bjarnason N, Henriksen E, Alexandersen P, Christgau S, Henriksen D, Christiansen C . Mechanism of circadian variation in bone resorption. Bone. 2002; 30(1):307-13. DOI: 10.1016/s8756-3282(01)00662-7. View

Borg G . Psychophysical bases of perceived exertion. Med Sci Sports Exerc. 1982; 14(5):377-81. View

Carter J, Lee D, Ranchordas M, Cole M . Perspectives of the barriers and enablers to nutritional adherence in professional male academy football players. Sci Med Footb. 2022; 7(4):394-405. DOI: 10.1080/24733938.2022.2123554. View

Christgau S . Circadian variation in serum CrossLaps concentration is reduced in fasting individuals. Clin Chem. 2000; 46(3):431. View

10.

Clowes J, Hannon R, Yap T, Hoyle N, Blumsohn A, Eastell R . Effect of feeding on bone turnover markers and its impact on biological variability of measurements. Bone. 2002; 30(6):886-90. DOI: 10.1016/s8756-3282(02)00728-7. View

11.

Collins J, Maughan R, Gleeson M, Bilsborough J, Jeukendrup A, Morton J . UEFA expert group statement on nutrition in elite football. Current evidence to inform practical recommendations and guide future research. Br J Sports Med. 2020; 55(8):416. DOI: 10.1136/bjsports-2019-101961. View

12.

Coutts A, Duffield R . Validity and reliability of GPS devices for measuring movement demands of team sports. J Sci Med Sport. 2008; 13(1):133-5. DOI: 10.1016/j.jsams.2008.09.015. View

13.

Dolan E, Varley I, Ackerman K, Pereira R, Elliott-Sale K, Sale C . The Bone Metabolic Response to Exercise and Nutrition. Exerc Sport Sci Rev. 2020; 48(2):49-58. DOI: 10.1249/JES.0000000000000215. View

14.

Dolan E, Dumas A, Keane K, Bestetti G, Freitas L, Gualano B . The Bone Biomarker Response to an Acute Bout of Exercise: A Systematic Review with Meta-Analysis. Sports Med. 2022; 52(12):2889-2908. DOI: 10.1007/s40279-022-01718-8. View

15.

Febbraio M, Pedersen B . Muscle-derived interleukin-6: mechanisms for activation and possible biological roles. FASEB J. 2002; 16(11):1335-47. DOI: 10.1096/fj.01-0876rev. View

16.

Goulding A . Risk factors for fractures in normally active children and adolescents. Med Sport Sci. 2007; 51:102-120. DOI: 10.1159/000103007. View

17.

Hall E, Larruskain J, Gil S, Lekue J, Baumert P, Rienzi E . An injury audit in high-level male youth soccer players from English, Spanish, Uruguayan and Brazilian academies. Phys Ther Sport. 2020; 44:53-60. DOI: 10.1016/j.ptsp.2020.04.033. View

18.

Hammond K, Sale C, Fraser W, Tang J, Shepherd S, Strauss J . Post-exercise carbohydrate and energy availability induce independent effects on skeletal muscle cell signalling and bone turnover: implications for training adaptation. J Physiol. 2019; 597(18):4779-4796. DOI: 10.1113/JP278209. View

19.

Hannon M, Carney D, Floyd S, Parker L, Mckeown J, Drust B . Cross-sectional comparison of body composition and resting metabolic rate in Premier League academy soccer players: Implications for growth and maturation. J Sports Sci. 2020; 38(11-12):1326-1334. DOI: 10.1080/02640414.2020.1717286. View

20.

Hannon M, Coleman N, Parker L, Mckeown J, Unnithan V, Close G . Seasonal training and match load and micro-cycle periodization in male Premier League academy soccer players. J Sports Sci. 2021; 39(16):1838-1849. DOI: 10.1080/02640414.2021.1899610. View