Biological Characterization and Genomic Analysis of a Novel Bacteriophage, MopsHU1, Infecting Morganella Psychrotolerans

Overview

Journal Arch Virol

Specialty Microbiology

Date 2024 Aug 17

PMID 39153099

Authors

Shogo Yamaki

Koji Yamazaki

Affiliations

Soon will be listed here.

Abstract

Morganella psychrotolerans is a histamine-producing bacterium that causes histamine poisoning. In this study, we isolated and characterized a novel phage, MopsHU1, that infects M. psychrotolerans. MopsHU1 is a podovirus with a limited host spectrum. Genomic analysis showed that MopsHU1 belongs to the family Autographiviridae, subfamily Studiervirinae, and genus Kayfunavirus. Comparative analysis revealed that the MopsHU1 genome is similar to those of Citrobacter phage SH3 and Cronobacter phage Dev2. Moreover, the Escherichia coli phage K1F genome is also similar, except for its tailspike gene sequence. These results expand our understanding of the Kayfunavirus phages that infect Morganella spp. Note: The nucleotide sequence data reported here are available in the DDBJ/EMBL/GenBank database under the accession number LC799501.

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References

Hungerford J . Histamine and Scombrotoxins. Toxicon. 2021; 201:115-126. DOI: 10.1016/j.toxicon.2021.08.013. View

Wang D, Yamaki S, Kawai Y, Yamazaki K . Histamine Production Behaviors of a Psychrotolerant Histamine-Producer, Morganella psychrotolerans, in Various Environmental Conditions. Curr Microbiol. 2020; 77(3):460-467. DOI: 10.1007/s00284-019-01853-y. View

Oliveira H, Pinto G, Oliveira A, Noben J, Hendrix H, Lavigne R . Characterization and genomic analyses of two newly isolated Morganella phages define distant members among Tevenvirinae and Autographivirinae subfamilies. Sci Rep. 2017; 7:46157. PMC: 5384007. DOI: 10.1038/srep46157. View

Yamaki S, Omachi T, Kawai Y, Yamazaki K . Characterization of a novel Morganella morganii bacteriophage FSP1 isolated from river water. FEMS Microbiol Lett. 2014; 359(2):166-72. DOI: 10.1111/1574-6968.12560. View

Yamaki S, Kuronuma S, Kawai Y, Yamazaki K . Inhibitory effect of a combination with novel jumbo bacteriophages ΦMV-1 and ΦMV-4 on Morganella morganii subsp. morganii growth and histamine accumulation. Int J Food Microbiol. 2019; 317:108457. DOI: 10.1016/j.ijfoodmicro.2019.108457. View

Zhu J, Rao X, Tan Y, Xiong K, Hu Z, Chen Z . Identification of lytic bacteriophage MmP1, assigned to a new member of T7-like phages infecting Morganella morganii. Genomics. 2010; 96(3):167-72. DOI: 10.1016/j.ygeno.2010.06.001. View

Jakociune D, Moodley A . A Rapid Bacteriophage DNA Extraction Method. Methods Protoc. 2019; 1(3). PMC: 6481073. DOI: 10.3390/mps1030027. View

Bankevich A, Nurk S, Antipov D, Gurevich A, Dvorkin M, Kulikov A . SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. 2012; 19(5):455-77. PMC: 3342519. DOI: 10.1089/cmb.2012.0021. View

Garneau J, Depardieu F, Fortier L, Bikard D, Monot M . PhageTerm: a tool for fast and accurate determination of phage termini and packaging mechanism using next-generation sequencing data. Sci Rep. 2017; 7(1):8292. PMC: 5557969. DOI: 10.1038/s41598-017-07910-5. View

10.

Aziz R, Bartels D, Best A, DeJongh M, Disz T, Edwards R . The RAST Server: rapid annotations using subsystems technology. BMC Genomics. 2008; 9:75. PMC: 2265698. DOI: 10.1186/1471-2164-9-75. View

11.

Besemer J, Lomsadze A, Borodovsky M . GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions. Nucleic Acids Res. 2001; 29(12):2607-18. PMC: 55746. DOI: 10.1093/nar/29.12.2607. View

12.

Altschul S, Gish W, Miller W, Myers E, Lipman D . Basic local alignment search tool. J Mol Biol. 1990; 215(3):403-10. DOI: 10.1016/S0022-2836(05)80360-2. View

13.

Chan P, Lowe T . tRNAscan-SE: Searching for tRNA Genes in Genomic Sequences. Methods Mol Biol. 2019; 1962:1-14. PMC: 6768409. DOI: 10.1007/978-1-4939-9173-0_1. View

14.

Aziz R, Ackermann H, Petty N, Kropinski A . Essential Steps in Characterizing Bacteriophages: Biology, Taxonomy, and Genome Analysis. Methods Mol Biol. 2017; 1681:197-215. DOI: 10.1007/978-1-4939-7343-9_15. View

15.

Moraru C, Varsani A, Kropinski A . VIRIDIC-A Novel Tool to Calculate the Intergenomic Similarities of Prokaryote-Infecting Viruses. Viruses. 2020; 12(11). PMC: 7694805. DOI: 10.3390/v12111268. View

16.

Ohtsubo Y, Ikeda-Ohtsubo W, Nagata Y, Tsuda M . GenomeMatcher: a graphical user interface for DNA sequence comparison. BMC Bioinformatics. 2008; 9:376. PMC: 2553346. DOI: 10.1186/1471-2105-9-376. View

17.

Turner D, Kropinski A, Adriaenssens E . A Roadmap for Genome-Based Phage Taxonomy. Viruses. 2021; 13(3). PMC: 8003253. DOI: 10.3390/v13030506. View

18.

Kajsik M, Oslanecova L, Szemes T, Hyblova M, Bilkova A, Drahovska H . Characterization and genome sequence of Dev2, a new T7-like bacteriophage infecting Cronobacter turicensis. Arch Virol. 2014; 159(11):3013-9. DOI: 10.1007/s00705-014-2173-5. View

19.

Klumpp J, Dunne M, Loessner M . A perfect fit: Bacteriophage receptor-binding proteins for diagnostic and therapeutic applications. Curr Opin Microbiol. 2022; 71:102240. DOI: 10.1016/j.mib.2022.102240. View

20.

Hamdi S, Rousseau G, Labrie S, Kourda R, Tremblay D, Moineau S . Characterization of Five Podoviridae Phages Infecting Citrobacter freundii. Front Microbiol. 2016; 7:1023. PMC: 4925675. DOI: 10.3389/fmicb.2016.01023. View