Quorum Sensing and Biofilm Attenuation by a Di-hydroxy Derivative of Piperlongumine (PL-18)

Overview

Journal Biofilm

Date 2024 Aug 16

PMID 39148892

Authors

Yael Schlichter Kadosh

Subramani Muthuraman

Khairun Nisaa

Anat Ben-Zvi

Danit Lisa Karsagi Byron

Marilou Shagan

Alexander Brandis

Tevie Mehlman

Jacob Gopas

Rajendran Saravana Kumar

Ariel Kushmaro

Affiliations

Soon will be listed here.

Abstract

Bacterial communication, Quorum Sensing (QS), is a target against virulence and prevention of antibiotic-resistant infections. 16 derivatives of Piperlongumine (PL), an amide alkaloid from L., were screened for QS inhibition. PL-18 had the best QSI activity. PL-18 inhibited the and QS systems of . PL-18 inhibited pyocyanin and rhamnolipids that are QS-controlled virulence elements. Iron is an essential element for pathogenicity, biofilm formation and resilience in harsh environments, its uptake was inhibited by PL-18. Pl-18 significantly reduced the biofilm biovolume including in established biofilms. PL-18-coated silicon tubes significantly inhibited biofilm formation. The transcriptome study of treated showed that PL-18 indeed reduced the expression of QS and iron homeostasis related genes, and up regulated sulfur metabolism related genes. Altogether, PL-18 inhibits QS, virulence, iron uptake, and biofilm formation. Thus, PL-18 should be further developed against bacterial infection, antibiotic resistance, and biofilm formation.

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