Balloon Compression-induced Spinal Cord Injury in Canines: a Large Animal Model for Spinal Cord Injury Research

Overview

Journal J Med Life

Specialty General Medicine

Date 2024 Aug 15

PMID 39144692

Authors

Yudha Mathan Sakti

Emir Riandika Samyudia

Deas Makalingga Emiri

Teguh Aryandono

Rahadyan Magetsari

Rusdy Ghazali Malueka

Ery Kus Dwianingsih

Affiliations

Soon will be listed here.

Abstract

Spinal cord injury (SCI) is a life-altering condition that severely impacts an individual's functional capabilities and has significant implications for both the individual and society. Large animal models are crucial for understanding the pathology and biomechanics of SCI. Dogs () are promising models for SCI research due to their anatomical and histopathological similarities to humans. Balloon compression is an established method for inducing controlled SCI in canines. In this study, we optimized a balloon compression procedure for inducing SCI in dogs, aiming to develop a reliable model for future in vivo studies. Our methodology successfully induced total motoric loss in canines, observed for seven days, a critical period for therapeutic interventions. Histopathological examinations using Luxol fast blue (LFB) staining revealed total demyelination in intralesional samples, confirming the structural damage caused by balloon compression. We concluded that a balloon compression model at the T10-T11 vertebral level, with an inflated balloon volume of 1.0 ml, induced SCI while minimizing the risk of balloon rupture. Longer duration of compression ensures total paralysis in this model, providing a platform for testing therapeutic interventions during the acute phase of SCI. The canine model generated consistent data and facilitated straightforward observational findings.

References

Fukuda S, Nakamura T, Kishigami Y, Endo K, Azuma T, Fujikawa T . New canine spinal cord injury model free from laminectomy. Brain Res Brain Res Protoc. 2005; 14(3):171-80. DOI: 10.1016/j.brainresprot.2005.01.001. View

Spitzbarth I, Moore S, Stein V, Levine J, Kuhl B, Gerhauser I . Current Insights Into the Pathology of Canine Intervertebral Disc Extrusion-Induced Spinal Cord Injury. Front Vet Sci. 2020; 7:595796. PMC: 7653192. DOI: 10.3389/fvets.2020.595796. View

Gorney A, Blau S, Dohse C, Griffith E, Williams K, Lim J . Mechanical and Thermal Sensory Testing in Normal Chondrodystrophoid Dogs and Dogs with Spinal Cord Injury caused by Thoracolumbar Intervertebral Disc Herniations. J Vet Intern Med. 2016; 30(2):627-35. PMC: 4913607. DOI: 10.1111/jvim.13913. View

Bergers E, Bot J, De Groot C, Polman C, Lycklama A Nijeholt G, Castelijns J . Axonal damage in the spinal cord of MS patients occurs largely independent of T2 MRI lesions. Neurology. 2002; 59(11):1766-71. DOI: 10.1212/01.wnl.0000036566.00866.26. View

Norenberg M, Smith J, Marcillo A . The pathology of human spinal cord injury: defining the problems. J Neurotrauma. 2004; 21(4):429-40. DOI: 10.1089/089771504323004575. View

Liau L, Looi Q, Chia W, Subramaniam T, Ng M, Law J . Treatment of spinal cord injury with mesenchymal stem cells. Cell Biosci. 2020; 10:112. PMC: 7510077. DOI: 10.1186/s13578-020-00475-3. View

Alizadeh A, Dyck S, Karimi-Abdolrezaee S . Traumatic Spinal Cord Injury: An Overview of Pathophysiology, Models and Acute Injury Mechanisms. Front Neurol. 2019; 10:282. PMC: 6439316. DOI: 10.3389/fneur.2019.00282. View

Sharif-Alhoseini M, Khormali M, Rezaei M, Hajighadery A, Khalatbari M, Safdarian M . Animal models of spinal cord injury: a systematic review. Spinal Cord. 2017; 55(8):714-721. DOI: 10.1038/sc.2016.187. View

Sheng S, Wang X, Xu H, Zhu G, Zhou Y . Anatomy of large animal spines and its comparison to the human spine: a systematic review. Eur Spine J. 2009; 19(1):46-56. PMC: 2899734. DOI: 10.1007/s00586-009-1192-5. View

10.

Teraguchi M, Yoshimura N, Hashizume H, Muraki S, Yamada H, Minamide A . Prevalence and distribution of intervertebral disc degeneration over the entire spine in a population-based cohort: the Wakayama Spine Study. Osteoarthritis Cartilage. 2013; 22(1):104-10. DOI: 10.1016/j.joca.2013.10.019. View

11.

Laitinen O, Puerto D . Surgical decompression in dogs with thoracolumbar intervertebral disc disease and loss of deep pain perception: A retrospective study of 46 cases. Acta Vet Scand. 2005; 46(1-2):79-85. PMC: 2202789. DOI: 10.1186/1751-0147-46-79. View

12.

Elmasry S, Asfour S, de Rivero Vaccari J, Travascio F . Effects of Tobacco Smoking on the Degeneration of the Intervertebral Disc: A Finite Element Study. PLoS One. 2015; 10(8):e0136137. PMC: 4547737. DOI: 10.1371/journal.pone.0136137. View

13.

Rossi G, Stachel A, Lynch A, Olby N . Intervertebral disc disease and aortic thromboembolism are the most common causes of acute paralysis in dogs and cats presenting to an emergency clinic. Vet Rec. 2020; 187(10):e81. DOI: 10.1136/vr.105844. View

14.

Araujo R, Firmino-Machado J, Correia P, Leitao-Marques M, Carvalho J, Silva M . The plantar reflex: A study of observer agreement, sensitivity, and observer bias. Neurol Clin Pract. 2018; 5(4):309-316. PMC: 5764473. DOI: 10.1212/CPJ.0000000000000155. View

15.

Courtine G, Bunge M, Fawcett J, Grossman R, Kaas J, Lemon R . Can experiments in nonhuman primates expedite the translation of treatments for spinal cord injury in humans?. Nat Med. 2007; 13(5):561-6. PMC: 3245971. DOI: 10.1038/nm1595. View

16.

Fjeld O, Grovle L, Helgeland J, Smastuen M, Solberg T, Zwart J . Complications, reoperations, readmissions, and length of hospital stay in 34 639 surgical cases of lumbar disc herniation. Bone Joint J. 2019; 101-B(4):470-477. DOI: 10.1302/0301-620X.101B4.BJJ-2018-1184.R1. View

17.

Venkatesh K, Ghosh S, Mullick M, Manivasagam G, Sen D . Spinal cord injury: pathophysiology, treatment strategies, associated challenges, and future implications. Cell Tissue Res. 2019; 377(2):125-151. DOI: 10.1007/s00441-019-03039-1. View

18.

Saadoun S, Jeffery N . Acute Traumatic Spinal Cord Injury in Humans, Dogs, and Other Mammals: The Under-appreciated Role of the Dura. Front Neurol. 2021; 12:629445. PMC: 7887286. DOI: 10.3389/fneur.2021.629445. View

19.

TARLOV I . Spinal cord compression studies. III. Time limits for recovery after gradual compression in dogs. AMA Arch Neurol Psychiatry. 1954; 71(5):588-97. View

20.

Henke D, Gorgas D, Doherr M, Howard J, Forterre F, Vandevelde M . Longitudinal extension of myelomalacia by intramedullary and subdural hemorrhage in a canine model of spinal cord injury. Spine J. 2015; 16(1):82-90. DOI: 10.1016/j.spinee.2015.09.018. View