Unveiling Potential Sex-determining Genes and Sex-specific Markers in Autotetraploid Carassius Auratus

Overview

Journal Sci China Life Sci

Specialties Biology
Science

Date 2024 Aug 13

PMID 39136860

Authors

Kun Zhang

Xu Huang

Chongqing Wang

Xidan Xu

Xiaowei Xu

Xiaoping Dong

Qingwen Xiao

Jinhai Bai

Yue Zhou

Zhengkun Liu

Xinyi Deng

Yan Tang

Siyang Li

Enkui Hu

Wanjing Peng

Ling Xiong

Qinbo Qin

Shaojun Liu

Affiliations

Soon will be listed here.

Abstract

Autotetraploid Carassius auratus is a stable hereditary autotetraploid fish resulting from the hybridization of Carassius auratus red var. (RCC, ♀) × Megalobrama amblycephala (BSB, ♂), containing four sets of RCC chromosomes. However, the molecular mechanism underlying the determination of sex in this species remains largely unknown. Currently, there lacks a full understanding of the molecular mechanisms governing sex determination and specific molecular markers to differentiate sex in this species. In this study, 25,801,677 SNPs (Single-nucleotide polymorphism) and 6,210,306 Indels (insertion-deletion) were obtained from whole-genome resequencing of 100 individuals (including 50 female and 50 male). Further identification confirmed the candidate chromosomes as Chr46B, with the sex-determining region located at Chr46B: 22,500,000-22,800,000 bp. Based on the male-specific insertion (26 bp) within the candidate sex-determining region, a pair of sex-specific molecular markers has been identified. In addition, based on the screening of candidate sex-determining region genes and RT-qPCR validation analysis, ADAM10, AQP9 and tc1a were identified as candidate sex-determining genes. These findings provide a robust foundation for investigating sex determination mechanisms in fish, the evolution of sex chromosomes, and the development of monosex populations.

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