Phytomediated Copper Oxide Nanoparticles Derived from the Fronds of Adiantum Venustum D.Don: Evaluation of Their Biomedical Potential

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 2024 Aug 12

PMID 39134909

Authors

Neetika Kimta

Ankush Chauhan

Sunil Puri

Amita Kumari

Rohit Sharma

Akshay Kumar

Dhriti Kapoor

Affiliations

Soon will be listed here.

Abstract

The green synthesis of copper oxide nanoparticles (CuO) mediated by crude ethanolic extract and the n-butanol fraction of Adiantum venustum represents a groundbreaking approach in nanotechnology, combining ecological sustainability with advanced functionality. This innovative method leverages the natural bioactive compounds present in A. venustum to produce CuO nanoparticles, which exhibit remarkable antioxidant, anti-inflammatory, antimicrobial, and anti-proliferative properties. The green synthesized nanoparticles were characterized using a variety of techniques, as XRD confirmed the crystalline nature of the CuO nanoparticles, with a crystallite size of 14.65 nm for CuO-C and 18.73 nm for CuO-B. The grain sizes of CuO-C (14.09 ± 0.17 nm) and CuO-B (67.88 ± 2.08 nm) were determined using transmission electron microscopy micrographs. Furthermore, the synthesized nanomaterial and the crude ethanolic extract, n-butanol fraction, were examined for their biological potentials namely antioxidant, anti-inflammatory, antimicrobial, and anti-proliferative activity against HeLa cancer cells. Among the synthesized nanomaterials, copper oxide nanoparticles synthesized by utilizing the n-butanol fraction have appeared as a potential biomedical agent. CuO-B has arisen as an antioxidant agent with IC values of 44.63 ± 0.49 µg/mL, 48.49 ± 0.17 µg/mL, and 35.39 ± 0.61 µg/mL for DPPH, FRAP, and reducing power assay, respectively. Furthermore, the significant antibacterial potential of CuO-B against gram-positive (S. aureus MIC 46.88 µg/mL) and gram-negative (K. pneumonia MIC 23.48 µg/mL) bacterial strains cannot be neglected either. Along with this, the IC value (138.07 µg/mL) of CuO-B against HeLa cells proved it to be a potential anticancerous agent. Hence, this novel approach emphasized that these synthesized nanoparticles have tremendous biological potential and can be applied to various fields of agriculture and biomedicine.

References

Mali S, Dhaka A, Githala C, Trivedi R . Green synthesis of copper nanoparticles using Willd. leaf extract and their photocatalytic and antifungal properties. Biotechnol Rep (Amst). 2020; 27:e00518. PMC: 7475076. DOI: 10.1016/j.btre.2020.e00518. View

Pugazhendhi A, Prabhu R, Muruganantham K, Shanmuganathan R, Natarajan S . Anticancer, antimicrobial and photocatalytic activities of green synthesized magnesium oxide nanoparticles (MgONPs) using aqueous extract of Sargassum wightii. J Photochem Photobiol B. 2018; 190:86-97. DOI: 10.1016/j.jphotobiol.2018.11.014. View

Shaheen T, Abd El Aty A . In-situ green myco-synthesis of silver nanoparticles onto cotton fabrics for broad spectrum antimicrobial activity. Int J Biol Macromol. 2018; 118(Pt B):2121-2130. DOI: 10.1016/j.ijbiomac.2018.07.062. View

Meftahi A, Samyn P, Geravand S, Khajavi R, Alibkhshi S, Bechelany M . Nanocelluloses as skin biocompatible materials for skincare, cosmetics, and healthcare: Formulations, regulations, and emerging applications. Carbohydr Polym. 2022; 278:118956. DOI: 10.1016/j.carbpol.2021.118956. View

Oves M, Rauf M, Aslam M, Qari H, Sonbol H, Ahmad I . Green synthesis of silver nanoparticles by plant extract and their antimicrobial and anticancer activities. Saudi J Biol Sci. 2022; 29(1):460-471. PMC: 8716933. DOI: 10.1016/j.sjbs.2021.09.007. View

Harish V, Tewari D, Gaur M, Yadav A, Swaroop S, Bechelany M . Review on Nanoparticles and Nanostructured Materials: Bioimaging, Biosensing, Drug Delivery, Tissue Engineering, Antimicrobial, and Agro-Food Applications. Nanomaterials (Basel). 2022; 12(3). PMC: 8839643. DOI: 10.3390/nano12030457. View

Barhoum A, Jeevanandam J, Rastogi A, Samyn P, Boluk Y, Dufresne A . Plant celluloses, hemicelluloses, lignins, and volatile oils for the synthesis of nanoparticles and nanostructured materials. Nanoscale. 2020; 12(45):22845-22890. DOI: 10.1039/d0nr04795c. View

Eddine Laouini S, Bouafia A, Soldatov A, Algarni H, Tedjani M, Ali G . Green Synthesized of Ag/AgO Nanoparticles Using Aqueous Leaves Extracts of L. and Their Azo Dye Photodegradation. Membranes (Basel). 2021; 11(7). PMC: 8306034. DOI: 10.3390/membranes11070468. View

Zhang X, Wang K, Liu M, Zhang X, Tao L, Chen Y . Polymeric AIE-based nanoprobes for biomedical applications: recent advances and perspectives. Nanoscale. 2015; 7(27):11486-508. DOI: 10.1039/c5nr01444a. View

10.

Noman M, Shahid M, Ahmed T, Tahir M, Naqqash T, Muhammad S . Green copper nanoparticles from a native Klebsiella pneumoniae strain alleviated oxidative stress impairment of wheat plants by reducing the chromium bioavailability and increasing the growth. Ecotoxicol Environ Saf. 2020; 192:110303. DOI: 10.1016/j.ecoenv.2020.110303. View

11.

Gaur M, Misra C, Yadav A, Swaroop S, O Maolmhuaidh F, Bechelany M . Biomedical Applications of Carbon Nanomaterials: Fullerenes, Quantum Dots, Nanotubes, Nanofibers, and Graphene. Materials (Basel). 2021; 14(20). PMC: 8538389. DOI: 10.3390/ma14205978. View

12.

Alhares H, Ali Q, Shaban M, M-Ridha M, Bohan H, Mohammed S . Rice husk coated with copper oxide nanoparticles for 17α-ethinylestradiol removal from an aqueous solution: adsorption mechanisms and kinetics. Environ Monit Assess. 2023; 195(9):1078. DOI: 10.1007/s10661-023-11689-6. View

13.

Fierascu I, Fierascu I, Brazdis R, Baroi A, Fistos T, Fierascu R . Phytosynthesized Metallic Nanoparticles-between Nanomedicine and Toxicology. A Brief Review of 2019's Findings. Materials (Basel). 2020; 13(3). PMC: 7040630. DOI: 10.3390/ma13030574. View

14.

Ghosh A, Chandra G . Biocontrol efficacy of Cestrum diurnum L. (Solanaceae: Solanales) against the larval forms of Anopheles stephensi. Nat Prod Res. 2006; 20(4):371-9. DOI: 10.1080/14786410600661575. View

15.

Mehmood N, Zubair M, Rizwan K, Rasool N, Shahid M, Ahmad V . Antioxidant, antimicrobial and phytochemical analysis of cichoriumintybus seeds extract and various organic fractions. Iran J Pharm Res. 2013; 11(4):1145-51. PMC: 3813158. View

16.

Benzie I, Strain J . The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996; 239(1):70-6. DOI: 10.1006/abio.1996.0292. View

17.

Banerjee S, Chanda A, Adhikari A, Das A, Biswas S . Evaluation of Phytochemical Screening and Anti Inflammatory Activity of Leaves and Stem of Mikania scandens (L.) Wild. Ann Med Health Sci Res. 2014; 4(4):532-6. PMC: 4160675. DOI: 10.4103/2141-9248.139302. View

18.

Soltan-Dallal M, Validi M, Douraghi M, Fallah-Mehrabadi J, Lormohammadi L . Evaluation the cytotoxic effect of cytotoxin-producing Klebsiella oxytoca isolates on the HEp-2 cell line by MTT assay. Microb Pathog. 2017; 113:416-420. DOI: 10.1016/j.micpath.2017.11.003. View

19.

Badawy A, Abdelfattah N, Salem S, Awad M, Fouda A . Efficacy Assessment of Biosynthesized Copper Oxide Nanoparticles (CuO-NPs) on Stored Grain Insects and Their Impacts on Morphological and Physiological Traits of Wheat ( L.) Plant. Biology (Basel). 2021; 10(3). PMC: 8002698. DOI: 10.3390/biology10030233. View

20.

Khokhra R, Bharti B, Lee H, Kumar R . Visible and UV photo-detection in ZnO nanostructured thin films via simple tuning of solution method. Sci Rep. 2017; 7(1):15032. PMC: 5678174. DOI: 10.1038/s41598-017-15125-x. View